Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 10,323 results for: Coli

Clear keywords
Showing: 4681 - 4720 of 10323 results

  3. p028g13-Gb1

    Plasmid
    #249632
    Purpose
    Clone 8xHis-residues in tandem with Gb1 polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Article
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with 8x His-residues in tandem with Gb1 polypeptide
    Expression
    Bacterial
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249632-plasmid-map-sequence-id-504978

    Enlarge

  4. p028g13-MBP

    Plasmid
    #249631
    Purpose
    Clone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Article
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with MBP polypeptide
    Expression
    Bacterial
    Mutation
    Silent mutation on Gly-20 to remove a BsaI recogn…
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249631-plasmid-map-sequence-id-504970

    Enlarge

  6. p-ArsRBS2-cre[LVA]-loxPP-syfp2

    Plasmid
    #252621
    Purpose
    Cre-recombinase based arsenite biosensor. ArsR promoter regulates cre expression, which excises two transcriptional terminators leading to cell fluorescence.
    Depositor
    Andrea Giometto
    Article
    Garabello et al bioRxiv [Preprint]. 2025 Sep 2:2024.10.0
    Insert
    ParsR-arsR-cre[LVA] + PrpsL-loxP-TT-loxP-syfp2
    Tags
    cre[LVA]
    Expression
    Bacterial
    Promoter
    ParsR
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    252621-plasmid-map-sequence-id-504557

    Enlarge

  8. p-cymR-cre[LVA]-loxPP-eyfp

    Plasmid
    #252620
    Purpose
    Cumate-inducible Cre recombinase; cumate activates P_cymRC to express cre[LVA], which excises loxP-flanked transcriptional terminators and activates eyfp. Use with Marionette Sensor Collection strains
    Depositor
    Andrea Giometto
    Article
    Garabello et al bioRxiv [Preprint]. 2025 Sep 2:2024.10.0
    Insert
    cymRAM + PcymRC-cre[LVA] + PW4-loxP-TT-loxP-eyfp
    Tags
    cre[LVA]
    Expression
    Bacterial
    Promoter
    PcymRC
    Available Since
    March 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    252620-plasmid-map-sequence-id-503365

    Enlarge

  15. pBA1751

    Plasmid
    #246066
    Purpose
    Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori
    Depositor
    Jennifer Doudna
    Article
    Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2
    Insert
    Panoptes locus
    Expression
    Bacterial
    Promoter
    pJEX promoter
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    246066-plasmid-map-sequence-id-491110

    Enlarge

  16. pBA1729

    Plasmid
    #246063
    Purpose
    Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori
    Depositor
    Jennifer Doudna
    Article
    Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2
    Insert
    Panoptes locus
    Expression
    Bacterial
    Promoter
    pTet promoter
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    246063-plasmid-map-sequence-id-490799

    Enlarge

  19. SBC015898

    Plasmid
    #226285
    Purpose
    Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.
    Depositor
    Rainer Breitling
    Article
    Chua et al Metab Eng Commun. 2024 May 15;18:e00237.
    Inserts
    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    Eugenol O-methyltransferase
    Expression
    Bacterial
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226285-plasmid-map-sequence-id-451912

    Enlarge

  25. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid
    #201534
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB3
    Use
    Synthetic Biology
    Mutation
    rhaS W190G
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201534-plasmid-map-sequence-id-406801

    Enlarge

  27. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Promoter
    P-LlacO-1
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200837-plasmid-map-sequence-id-405511

    Enlarge

  28. pTP-PCR C1

    Plasmid
    #189618
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    189618-plasmid-map-sequence-id-401652

    Enlarge

  29. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201533-plasmid-map-sequence-id-398133

    Enlarge

  30. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201535-plasmid-map-sequence-id-398128

    Enlarge

  31. pVioCD53

    Plasmid
    #191130
    Purpose
    Expression of vioC, truncated vioD and tdMCP form a J23105 constitutive promoter
    Depositor
    Ariel Lindner
    Article
    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.
    Inserts
    vioC (vioC Synthetic)
    vioD truncated
    dMCP off-frame
    Use
    Synthetic Biology
    Mutation
    C-terminal truncation from 264th amino acid
    Promoter
    J23105 constitutive promoter
    Available Since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191130-plasmid-map-sequence-id-382518

    Enlarge

  33. pTP-PCR C4

    Plasmid
    #189621
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, and minor additional mutat…
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189621-plasmid-map-sequence-id-379021

    Enlarge

  34. pTP-PCR C2

    Plasmid
    #189619
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189619-plasmid-map-sequence-id-379034

    Enlarge

Showing: 4681 - 4720 of 10323 results