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miniCMV eGFP hPGK BSD

Plasmid

#188519

Purpose

Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cre)

Plasmid

#174863

Purpose

Expresses human Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by HsPeg10 UTRs

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-lexAp65

Plasmid

#138393

Purpose

RMCE vector for generating C-terminal T2A-lexA-p65 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-lexAp65

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SHP2 WT

Plasmid

#8381

Depositor

Ben Neel

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only

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scFv-sfGFP-DNMT3A1

Plasmid

#102278

Purpose

The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

Depositor

Margaret Goodell

Article

Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

Insert

DNMT3A1 (DNMT3A Human)

Use

Lentiviral

Tags

scFV-sfGFP

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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phage-cmv-cfp-24xms2

Plasmid

#40651

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xMBS

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pMHG-W57* reporter

Plasmid

#72850

Purpose

GFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.

Depositor

Daniel Liebler

Article

Halvey et al FEBS Open Bio. 2012 Dec;2:56-59. Epub 20

Insert

GFP

Expression

Mammalian

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

Plasmid

#162377

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pN1-GCaMP6m-XC

Plasmid

#111543

Purpose

Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

Depositor

Xiaodong Liu

Article

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XC

Expression

Mammalian

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-PRDX1

Plasmid

#38086

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PRDX1 peroxiredoxin 1 (PRDX1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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