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eGFP-KIF1A

Plasmid

#172206

Purpose

Expresses KIF1A tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG R2pH-LAMP1-3xFLAG

Plasmid

#157940

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Tags

3XFLAG, mCherry, and pHluorin

Expression

Mammalian

Promoter

Chick beta actin pomoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry-hCdt1(1-100)ΔCy

Plasmid

#193759

Purpose

Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

mCherry-hCdt1(1-100)ΔCy (CDT1 Human)

Use

Lentiviral

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

EF1a

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJA089

Plasmid

#128518

Purpose

Expresses human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV-RT

Plasmid

#181799

Purpose

MCP-M-MLV-RT (MS2-N55K-MMLVRT)

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MCP-M-MLV-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-FURIN-G418

Plasmid

#158462

Purpose

Constitutive lentiviral expression of human FURIN.

Depositor

Alejandro Chavez, Sho Iketani

Insert

FURIN (FURIN Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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MR015-H11-L2-TALEN

Plasmid

#51554

Purpose

Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

H11-L2 TALEN

Use

TALEN

Expression

Mammalian

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1

Plasmid

#204558

Purpose

Expresses GST-tagged UFBP1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

none

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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