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S. rosetta Col+ cDNA amplified library

Pooled Library

#62844

Purpose

amplified library of Salpingoeca rosetta from a colony rich culture which included the colony inducing bacteria Algoriphagus machipongonensis

Depositor

Nicole King

Article

Fairclough et al Genome Biol. 2013 Feb 18;14(2):R15. doi:

Expression

Bacterial

Species

Salpingoeca rosetta

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
CMV-REX-GECO0.9

Plasmid

#61247

Purpose

Expresses REX-GECO0.9 in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO0.9

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only
His-Tev-Nedd4L

Plasmid

#186806

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-Nedd4L (NEDD4L Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F263-OKT3

Plasmid

#190680

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-XL-s2193

Plasmid

#166749

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL (BCL2L1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

Human ferritin

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSB116 - pL2_pSB90_fLUC-I_P19

Plasmid

#123191

Purpose

binary plant vector for transient TBSV P19 and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::P19::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-F-PA1

Plasmid

#40618

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

PA1 (PAGR1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pCINeo-hFX-HA

Plasmid

#14976

Depositor

Michael Ristow

Article

Schulz et al J Biol Chem. 2006 Jan 13. 281(2):977-81.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-4T2 SHP2 D61A

Plasmid

#8323

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pLX304-ARNT

Plasmid

#203578

Purpose

Expresses wild-type ARNT in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

Insert

ARNT (ARNT Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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RINS1(mut)

Plasmid

#107291

Purpose

Plasmid for the expression of the inactive mutant of RINS1 sensor

Depositor

Dmytro Yushchenko

Article

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Insert

RINS1(mut)

Tags

sfGFP

Expression

Mammalian

Mutation

two Arg/Ser mutations at the beginning and in the…

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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WT C-His Ana GvpC in pET28a

Plasmid

#85732

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJKR-L-cdaR

Plasmid

#62558

Purpose

Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEZYpci

Plasmid

#18707

Purpose

pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Expression

Yeast

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pFUS_A2B

Plasmid

#43850

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pXP622

Plasmid

#26849

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pAWPSG-Po-nCas9-BE

Plasmid

#195740

Purpose

Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

Depositor

Seung-Goo Lee

Article

Jeong et al Chem Eng J, 2023

Insert

Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Po (phoenol-inducible promoter)

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

Plasmid

#192193

Purpose

Lenti-BCMA-CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

Plasmid

#62459

Purpose

expresses his-tagged C288S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 288 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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