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We narrowed to 255 results for: petD;

Showing: 221 - 240 of 255 results

  1. pCali2

    Plasmid
    #86119
    Purpose
    Multiplex temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA36
    mWasabi
    TcI
    mCherry
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T and D341A, P60L, D135V, K187R, K202I, L208Q
    Promoter
    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI
    Available since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86119-plasmid-map-sequence-id-173197

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  2. pThermeleon

    Plasmid
    #86120
    Purpose
    Bandpass temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…
    Promoter
    pR/pL, pR/pL, pLacI, and pTlpA
    Available since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86120-plasmid-map-sequence-id-173201

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  6. pDC146

    Plasmid
    #169366
    Purpose
    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Depositor
    Axel Brunger
    Article
    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45
    Insert
    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Use
    Tags
    6 his tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169366-plasmid-map-sequence-id-333023

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  13. pAM59

    Plasmid
    #227655
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, Δ78–81
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227655-plasmid-map-sequence-id-455288

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  14. pAM57

    Plasmid
    #227654
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, V80A
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227654-plasmid-map-sequence-id-455272

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  15. pAM54

    Plasmid
    #227653
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, L132A
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227653-plasmid-map-sequence-id-455251

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  16. pAM71

    Plasmid
    #227621
    Purpose
    pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)
    Depositor
    Andreas Martin
    Article
    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    His-Prescission
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 24-160-GGSGSG-192-221
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227621-plasmid-map-sequence-id-455206

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  17. pTcI38-PRM-wasabi

    Plasmid
    #195554
    Purpose
    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38
    Depositor
    Mikhail Shapiro
    Article
    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2
    Inserts
    mWasabi
    TcI38
    Use
    Tags
    6x His and AANDENYADAS degradation tag
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    195554-plasmid-map-sequence-id-426585

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  19. pDC183

    Plasmid
    #106415
    Purpose
    Co-expresses trans SNARE complex with pQZ210 in E. coli
    Depositor
    Axel Brunger
    Article
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    SNAP25 fragment 141-204 (Snap25 Synthetic, Rat)
    SNAP25 Fragment 7-83 (Snap25 Synthetic, Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fragment 141-204, Codon optimization and Fragment…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106415-plasmid-map-sequence-id-204169

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Showing: 221 - 240 of 255 results