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Showing: 221 - 240 of 19140 results

  4. pTwist-SARS-CoV-2 Spike-deltaC18 (Mockingbird)

    Plasmid
    #212411
    Purpose
    Encodes SARS-CoV-2 spike G142S,E180V,D614G,Q677H for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike G142S,E180V,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G142S,E180V,D614G,Q677H
    Promoter
    CAG
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212411-plasmid-map-sequence-id-431643

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  7. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119190-plasmid-map-sequence-id-232293

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  9. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

    Plasmid
    #174639
    Purpose
    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-GFP-ppKinVIb(861-1321)
    Use
    Tags
    SSPB(micro)-GFP
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174639-plasmid-map-sequence-id-346670

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  10. Desmoplakin II donor only control (FL-based tension sensor)

    Plasmid
    #118723
    Purpose
    The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted FL-based tension sensor module after aa1…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118723-plasmid-map-sequence-id-230442

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  12. Anti-LRRK2/Dardarin kinase, C-terminus [N241A/58R]

    Plasmid
    #220411
    Purpose
    Mammalian Expression Plasmid of anti-LRRK2/Dardarin kinase, C-terminus (Human) IgG2a R-mAb. Derived from hybridoma N241A/58
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-LRRK2/Dardarin kinase, C-terminus (Homo sapiens) recombinant mouse monoclonal antibody. (LRRK2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220411-plasmid-map-sequence-id-435564

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  13. pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

    Plasmid
    #174641
    Purpose
    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-ppKin14VIb(861-1321)
    Use
    Tags
    SSPB(micro)-Venus
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    174641-plasmid-map-sequence-id-355829

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  14. Desmoplakin II donor only control (FL-based no force control)

    Plasmid
    #118722
    Purpose
    The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118722-plasmid-map-sequence-id-230466

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  15. CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid
    #165435
    Purpose
    Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
    Use
    Tags
    ShadowY, Clover(T153M/F223R)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165435-plasmid-map-sequence-id-325982

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  16. pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174640
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
    Use
    Tags
    SSPB(micro)-GFP
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174640-plasmid-map-sequence-id-347051

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  17. pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174643
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
    Use
    Tags
    SSPB(nano)-GFP
    Expression
    Mammalian
    Mutation
    EGFP: Met1Del; ppKin14VIb(aa861-1321)
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174643-plasmid-map-sequence-id-346994

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  18. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid
    #119189
    Purpose
    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119189-plasmid-map-sequence-id-230531

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  19. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119188-plasmid-map-sequence-id-230532

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Showing: 221 - 240 of 19140 results