We narrowed to 1,145 results for: cas12

1. pJCC_058 FnCas12a D917A

   Plasmid

   #171667

   Purpose

   For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a D917A
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   changed aspartate 917 to alanine

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET28-FnCas12a-EP16

   Plasmid

   #183636

   Purpose

   PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

   Depositor

   Yunzi Luo

   Article

   Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

   Insert

   FnCas12a-EP16
   

   Use

   CRISPR

   Tags

   6×His Tag

   Expression

   Bacterial

   Mutation

   N607R, K613V, N617R, K180S, K660R, D616N

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-BsCas12a-2AeGFP

   Plasmid

   #123633

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   BsCas12a
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-PrCas12a-2AeGFP

   Plasmid

   #123636

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   PrCas12a
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-BsCas12b-2AeGFP

   Plasmid

   #121948

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   BsCas12b
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJH031_proD_LbCas12a-gRNA-GFP_CAM

   Plasmid

   #183075

   Purpose

   Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ292 (AaCas12b)-D570A-TV

   Plasmid

   #136380

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D570A

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis-MBP-huPcCas12a

   Plasmid

   #174681

   Purpose

   Expresses 6xHis-MBP tagged PcCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPcCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6xHis-MBP-huCMtCas12a

   Plasmid

   #174680

   Purpose

   Expresses 6xHis-MBP tagged CMtCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huCMtCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-huPdCas12a

    Plasmid

    #174682

    Purpose

    Expresses 6xHis-MBP tagged PdCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPdCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYC-OspCas12c-noncoding

    Plasmid

    #120878

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-OspCas12
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC_hfnCas12a-Nlux_crRNA NR1

    Plasmid

    #176244

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Ribosomal subunit

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-AmCas12b-2AeGFP

    Plasmid

    #121947

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. FnoCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126644

    Purpose

    Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIBR2.0-818-Cas12a-NT

    Plasmid

    #235168

    Purpose

    Expresses the SIBR2.0-818-Cas12a system and a NT crRNA

    Depositor

    Constantinos Patinios

    Article

    Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

    Insert

    SIBR2.0-818-Cas12a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid

    #236041

    Purpose

    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid

    #236039

    Purpose

    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. UAST-uMCas12a+nls2x

    Plasmid

    #230909

    Purpose

    inducible expression of Cas12a+ with Gal4.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-LbCas12a-8xNLS

    Plasmid

    #209020

    Purpose

    Lentiviral vector expressing human codon-optimized LbCas12a with 8xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    LbCas12a-8xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dCas12a-AID

    Plasmid

    #193647

    Purpose

    Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hAID, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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