We narrowed to 6,625 results for: PROC

1. pAcBac2.tR4-OMeYRS/GFP*

   Plasmid

   #50831

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Tyr tRNA cassette
   EGFP*
   OMeYRS
   two copy Tyr tRNA cassette
   

   Use

   Baculoviral

   Tags

   His, Myc-6xHis, and WPRE

   Expression

   Insect

   Mutation

   Y39TAG and able to charge various unnatural amino…

   Promoter

   CAG, CMV, and U6 and H1

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-puro-tetON-shRFP

   Plasmid

   #110940

   Purpose

   Tet-inducible shRNA targeting RFP

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shRFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-B7

   Plasmid

   #135509

   Purpose

   Mammalian expression of myc-tagged HLA-B*07:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-B*07:02 (HLA-B Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro HA PIK3CA H1047R

   Plasmid

   #12524

   Purpose

   Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1047R. Tumor-derived allele.

   Promoter

   Available since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET32a-HD46Q

   Plasmid

   #11515

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 46Q glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 46 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. ODD-Luciferase-pcDNA3

   Plasmid

   #18965

   Depositor

   William Kaelin

   Article

   Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

   Insert

   ODD-Firefly Luciferase (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HIF1a ODD sequence fused in frame to luciferase O…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. H2B-mGreenLantern

   Plasmid

   #164464

   Purpose

   Localization of mGreenLantern fluorescent protein to histone 2B

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Human Histone 2B (D26G and V119I mutations)

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDG1-PHP.eB

   Plasmid

   #220799

   Purpose

   AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   E4, E2A, VA, AAV2 rep and PHP.eB cap genes
   

   Use

   AAV; Aav helper vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3-N90-beta-catenin

   Plasmid

   #31785

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-90- Constitutitvely Active

   Promoter

   EF1alpha

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5-Flag-XBP1s

    Plasmid

    #63680

    Purpose

    Mammalian expression of Flag tagged XBP1s

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    spliced

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET32a-HD25Q

    Plasmid

    #11508

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 25 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 25 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-mGreenLantern

    Plasmid

    #164469

    Purpose

    Constitutive CAG-driven expression of mGreenLantern in animals using AAV

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CAG

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneo-lambdaN-HA-HsEDC4

    Plasmid

    #66597

    Purpose

    Expresses lambdaN-HA-tagged EDC4 in mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC4 (EDC4 Human)
    

    Use

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2Bc-T MBP_BoMoC(ed)_6xH

    Plasmid

    #186461

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1036 pcDNA3 Myr HA Akt1

    Plasmid

    #9008

    Purpose

    Mammalian expression of human Akt1 with myr, HA tags

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV4 p50

    Plasmid

    #21965

    Depositor

    Warner Greene

    Article

    Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

    Insert

    NF-kB p50 subunit (NFKB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. TagRFP-T-EEA1

    Plasmid

    #42635

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. EXT1- D164A-pLJC2-3XFLAG

    Plasmid

    #215828

    Purpose

    Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-FLEX-hKCNQ3-P2A-EGFP

    Plasmid

    #209195

    Purpose

    Cre-dependent expression of Kcnq3 and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCEP4-FLAG-TAPBPR

    Plasmid

    #135500

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    Luminal FLAG epitope tag and Signal peptide from …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YFP NLS Beta-Actin

    Plasmid

    #60613

    Purpose

    Encodes YFP and NLS tagged beta-actin

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pORTMAGE-4

    Plasmid

    #72679

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLNCX myr Akt1

    Plasmid

    #17245

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    Myr

    Expression

    Mammalian

    Mutation

    Constitutively active

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti PGK FKBP12(F36V)-OGT (Neo)

    Plasmid

    #154294

    Purpose

    Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMGF182

    Plasmid

    #97006

    Purpose

    Expresses GFP-CHMP7 in mammalian cells under a crippled promoter

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    CHMP7 (CHMP7 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV delta 5

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHD-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199203

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEXPqcxip-hFTSJ3-FLAG

    Plasmid

    #165463

    Purpose

    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    

    Use

    Retroviral; Gateway

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/MSV, 5'LTR

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 iCasper T2A HO1

    Plasmid

    #64278

    Purpose

    Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge