We narrowed to 22,480 results for: SCR

1. NODAL-T7TS

   Plasmid

   #115250

   Purpose

   For in vitro transcription of human NODAL open reading frame RNA

   Depositor

   Lynne Postovit

   Article

   Findlay et al (2018) bioRxiv 276170

   Insert

   NODAL (NODAL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-foxd4l1.1

   Plasmid

   #185493

   Purpose

   ISH probe for X laevis foxd4

   Depositor

   Sally Moody

   Article

   Sullivan et al Dev Biol. 2001 Apr 15. 232(2):439-57.

   Insert

   foxd4l1.1 S homeolog
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-foxd4l1.1

   Plasmid

   #185494

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Sullivan et al Dev Biol. 2001 Apr 15. 232(2):439-57.

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYY_S1_400

   Plasmid

   #170470

   Purpose

   To amplify transcription template containing LacI operators

   Depositor

   Laura Finzi

   Article

   Kovari et al Methods Mol Biol. 2018;1665:317-340. doi

   Insert

   T7A1, Os, O1, lambda t1, in lacZ fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7A1

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS_1N_400

   Plasmid

   #170476

   Purpose

   To amplify transcription template containing LacI operators

   Depositor

   Laura Finzi

   Article

   Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

   Insert

   T7A1, O1, O-, Lambda t1 in lacZ fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7A1

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pELF4.1.0-gDNA

   Plasmid

   #112467

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELF4

   Depositor

   Kevin White

   Insert

   ELF4 (ELF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pE2F5.1.0-gDNA

   Plasmid

   #112447

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

   Depositor

   Kevin White

   Insert

   E2F5 (E2F5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHOXC13-donor

   Plasmid

   #113827

   Purpose

   CRISPR donor plasmid to tag human transcription factor HOXC13 with GFP

   Depositor

   Kevin White

   Insert

   HOXC13 homology arms flanking EGFP-IRES-Neo cassette (HOXC13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGLIS3-donor

   Plasmid

   #113812

   Purpose

   CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP

   Depositor

   Kevin White

   Insert

   GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   rs10118031, rs10973702, rs912257

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSRF-donor

    Plasmid

    #112362

    Purpose

    CRISPR donor plasmid to tag human transcription factor SRF with GFP

    Depositor

    Kevin White

    Insert

    SRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:6:43178470:C>A, hg38:6:43178514:G>A

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBP1-donor

    Plasmid

    #112336

    Purpose

    CRISPR donor plasmid to tag human transcription factor HBP1 with GFP

    Depositor

    Kevin White

    Insert

    HBP1 homology arms flanking EGFP-IRES-Neo cassette (HBP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZNF785-donor

    Plasmid

    #112317

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP

    Depositor

    Kevin White

    Insert

    ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:16:30582292:A>T, hg38:16:30582302:A>T,…

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZNF354B-donor

    Plasmid

    #112309

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP

    Depositor

    Kevin White

    Insert

    ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:5:178884427:G>A, hg38:5:178883445:C>T,…

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTOPO-rDNA-NTS

    Plasmid

    #23992

    Depositor

    Susan Baserga

    Article

    Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

    Insert

    rDNA NTS
    

    Use

    Bacterial cloning - topo

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pDD2045

    Plasmid

    #101379

    Purpose

    Name: pBluescript-empty vector. pBluescript-LANApi,K14 (pDD2000) bidirectional empty vector for backbone only.

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tet-inducible Luciferase reporter

    Plasmid

    #64127

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetOx13-CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI152-pLenti-SiT-Cas12a

    Plasmid

    #128405

    Purpose

    Express both AsCas12a and CRISPR arrays on single transcripts

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-Triplex
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    pTPGI (galactose+aTc inducible)

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GAL4UAS

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBbS9a-RFP

    Plasmid

    #126172

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23113

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pG12-SEAP

    Plasmid

    #170271

    Purpose

    Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    12xUAS with TATA-box minimal promoter

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBbB19k-GFP

    Plasmid

    #126135

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. wg186Fluc

    Plasmid

    #177436

    Purpose

    In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

    Depositor

    Wendy Gilbert

    Article

    Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

    Insert

    Firefly luciferase
    

    Use

    Luciferase; For in vitro transcription

    Tags

    None

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CFP-tTA-ER-C3

    Plasmid

    #134569

    Purpose

    Expresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

    Depositor

    Susan Janicki

    Article

    Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

    Insert

    tTA-ER
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116954

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. dCas9-AsLOV2x3-NLSx3

    Plasmid

    #231566

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    dCas9-AsLOV2x3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbB19c-GFP

    Plasmid

    #126139

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBbA13c-RFP

    Plasmid

    #126098

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LeGO-iG2-wPRE-pA

    Plasmid

    #60489

    Purpose

    eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)

    Depositor

    Boris Fehse

    Article

    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

    Insert

    IRES-eGFP; wPRE; BGH-pA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV_dCas9_VP64

    Plasmid

    #49015

    Purpose

    encodes human-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (human-codon-optimized)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pG-HIV-LRT

    Plasmid

    #104592

    Purpose

    Standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    HIV-1 late reverse transcripts
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBbB9a-GFP

    Plasmid

    #126112

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23113

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LentiUniversal-Puro

    Plasmid

    #127749

    Purpose

    Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)

    Depositor

    Giulio Draetta

    Article

    Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pELK3.1.0-gDNA

    Plasmid

    #112418

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

    Depositor

    Kevin White

    Insert

    ELK3 (ELK3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Enhanced Repressor Domain for TAL Effector (SID4X)

    Plasmid

    #43882

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    SID4X Domain
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pBbB12a-GFP

    Plasmid

    #126115

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23151

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge