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pcDNA-Sga-Cas9

Plasmid

#227005

Purpose

Mammalian expression of Nuclease active S. gallolyticus Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sin-Cas9

Plasmid

#227006

Purpose

Mammalian expression of Nuclease active S. iniae Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Spa-Cas9

Plasmid

#227007

Purpose

Mammalian expression of Nuclease active S. parasanguinis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MZB215

Plasmid

#221767

Purpose

Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf6 (ARF6 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q67L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB218

Plasmid

#221769

Purpose

Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf4 (ARF4 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB219

Plasmid

#221770

Purpose

Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf5 (ARF5 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB220

Plasmid

#221771

Purpose

Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arl1 (ARL1 Human)

Tags

CPD

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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RcSxph

Plasmid

#194018

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Tags

His10 and mEGFP,

Promoter

Polyhedrin

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspB

Plasmid

#215400

Purpose

Cm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspB

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspA2

Plasmid

#215399

Purpose

Cm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)

Depositor

Gitta Coaker

Article

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspA2

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MYD88-ts3

Plasmid

#174282

Purpose

MYD88 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MYD88 shRNA (MYD88 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ZC3HAV1-ts1

Plasmid

#174289

Purpose

ZC3HAV1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ZC3HAV1 shRNA (ZC3HAV1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ZC3HAV1-ts2

Plasmid

#174290

Purpose

ZC3HAV1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ZC3HAV1 shRNA (ZC3HAV1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ZC3HAV1-ts3

Plasmid

#174291

Purpose

ZC3HAV1 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ZC3HAV1 shRNA (ZC3HAV1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MOV10-ts3

Plasmid

#174279

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MYD88-ts1

Plasmid

#174280

Purpose

MYD88 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MYD88 shRNA (MYD88 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-MYD88-ts2

Plasmid

#174281

Purpose

MYD88 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MYD88 shRNA (MYD88 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-IFI16-ts1

Plasmid

#174256

Purpose

IFI16 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

IFI16 shRNA (IFI16 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-miR30-IFI16-ts2

Plasmid

#174257

Purpose

IFI16 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

IFI16 shRNA (IFI16 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge