We narrowed to 2,744 results for: ubiquitin
-
Plasmid#225938PurposeLentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only
-
pRGEB32-CrU6-2-UBI
Plasmid#226707PurposeFor CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.DepositorInsertCrU6-2
UseCRISPRAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD
Plasmid#199447PurposeExpession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signalDepositorInsertdSaCas9-10xGCN4
UseLentiviralExpressionMammalianPromoterhuman ubiquitin C (UBC)Available SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Usp5
Plasmid#174873PurposeCRISPR vector for generating Usp5 STREAMING-tag KI cellDepositorInsertsgRNA for mouse Usp5 (Usp5 Synthetic)
UseCRISPRAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97A-IRES-EGFP
Plasmid#183457PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97E-IRES-EGFP
Plasmid#183458PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G
Plasmid#169960PurposeMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)DepositorAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-UBC9-sh3
Plasmid#168987PurposeExpresses RFP cDNA and UBC9 shRNA 3 in mammalian cellsDepositorInsertUbiquitin Conjugating Enzyme 9
ExpressionMammalianPromoterUbc promoterAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G
Plasmid#169959PurposeMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E
Plasmid#169961PurposeMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G
Plasmid#170119PurposeMammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP
Plasmid#58490PurposeExpresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltageDepositorInsertArchaerhodopsin-3 w/ D95H point mutation fused to eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Aspartic Acid 95 to GlutaminePromoterUbiquitinAvailable SinceAug. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
UbiGate Components (set1)
Plasmid Kit#1000000144PurposeUbiGate Components (set 1) enables the reconstitution of the ubiquitination cascades of different organisms, including A. thaliana, in E. coli by 'Golden Gate' cloning.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypeBacterial ExpressionCloning TypeGolden GateAvailable SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Flag-Rheb
Plasmid#165031Purposeexpression of Flag tagged Rheb protein in mammalian cellsDepositorAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Flag-Rheb-4Rs
Plasmid#165022Purposeexpression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)DepositorInsertRheb-4Rs (RHEB Human)
UseLentiviralTagsflagExpressionMammalianMutationchange lysine residues on position 109, 135, 151,…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Flag-Rheb-12Rs
Plasmid#165023Purposeexpression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).DepositorInsertRheb-12Rs (RHEB Human)
UseLentiviralTagsflagExpressionMammalianMutationAll the lysine residues except K19 and K120 are c…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPSwe-T2A-mCherry
Plasmid#190804PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherryDepositorAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-C-prM-E-NS1
Plasmid#175281Purposelentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.DepositorInsertC-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
UseLentiviralTagsIRES EGFPPromoterhuman ubiquitin CAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSS02:cyt-Peredox-mCherry
Plasmid#161743Purposecytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plantsDepositorInsertcyt-Peredox-mCherry
ExpressionPlantPromoterUbiquitin 10Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
TagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only