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pHAGE-PIK3CA

Plasmid

#116771

Purpose

Lentiviral expression of PIK3CA

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLNCX2-mEGFP-VPS4A

Plasmid

#116924

Purpose

expresses mEGFP-VPS4A in mammalian cells

Depositor

Sanford Simon

Publication

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

silent mutation in position 4059 C to T

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Tau (P301L)-VC

Plasmid

#87633

Purpose

expresses hTau fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAPT (MAPT Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Mutation P301L

Promoter

CMV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJYS2_crtYf

Plasmid

#85544

Purpose

Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72667

Purpose

Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Publication

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pICH41780

Plasmid

#48019

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pT7-EGFP-C1-HsTNRC6A

Plasmid

#25035

Depositor

Elisa Izaurralde

Publication

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP

Expression

Mammalian

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-5FLAG-hphMX6

Plasmid

#19342

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

hphMX6

Expression

Yeast

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-5FLAG-hphMX6

Plasmid

#19342

Depositor

Eishi Noguchi

Publication

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

hphMX6

Expression

Yeast

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSFDuet-1_VNp6-mNeongreen

Plasmid

#182388

Purpose

Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 matrix (M )

Expression

Mammalian

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro

Plasmid

#141377

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles

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SP-GFP-CD274-NU

Plasmid

#121139

Purpose

Mammalian expression of GFP tagged CD274

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CD274 (CD274 Human)
CD274 (CD274 Human)

Expression

Mammalian

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGWB533

Plasmid

#74872

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NLS-dCas9-trCIB1

Plasmid

#64119

Purpose

Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-trCIB1 fusion

Tags

HA and NLS

Expression

Mammalian

Mutation

dCas9 (D10A H840A, catalytically inactive), trCIB…

Promoter

CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJA58

Plasmid

#59933

Purpose

gRNA for cleavage at dpy-10(cn64) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

dpy-10(cn64) gRNA

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET28a-SpySrtA

Plasmid

#51139

Purpose

expression plasmid for N-terminal his tagged S.pyogenes SrtA

Depositor

Hidde Ploegh

Publication

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.pyogenes SrtA

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-80

Promoter

T7

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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