We narrowed to 59,421 results for: cloning

1. pHHYTK123

   Plasmid

   #200130

   Purpose

   Type 7 part for Modular Cloning in Yeast

   Depositor

   Johannes Herrmann

   Article

   Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

   Insert

   pRS CEN/ARS
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-TARG1

   Plasmid

   #172577

   Purpose

   For gateway cloning of C-terminal tagged TARG1 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Use

   Gateway cloning

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR/zeo-TARG1

   Plasmid

   #172574

   Purpose

   For gateway cloning of N-terminal tagged TARG1 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Use

   Gateway cloning

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCm No carrier NikJC199U

   Plasmid

   #174361

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223 SCAF8_isoD

   Plasmid

   #122467

   Purpose

   pDONR for Gateway cloning

   Depositor

   Jesper Svejstrup

   Article

   Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

   Insert

   SCAF8 isoform D (SCAF8 Human)
   

   Use

   Gateway cloning

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGF004

   Plasmid

   #48845

   Purpose

   Provides a D-alanine resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   p35S::D-AlanineR::t35S
   

   Use

   Golden gate compatible cloning vector

   Mutation

   internal BsaI recognition site in 35S promoter re…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNH052

   Plasmid

   #42158

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   YFP-RT-YFP
   

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNH054

   Plasmid

   #42160

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mTFP1-RT-mTFP1
   

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNH039

   Plasmid

   #42151

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   CFP
   

   Available Since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNH058

    Plasmid

    #42161

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    TAP Tag::mTFP1
    

    Available Since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Tet3G blasticidin

    Plasmid

    #128061

    Purpose

    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO-neo (1795)

    Plasmid

    #41000

    Depositor

    Jonathon Pines

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid

    #223952

    Purpose

    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Karla Feijs, Roko Žaja

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT-TOgw-N-mCherry

    Plasmid

    #223948

    Purpose

    Gateway cloning of genes of interest with an N-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Karla Feijs, Roko Žaja

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSaGuide

    Plasmid

    #64710

    Purpose

    Cloning and expression of Staphylococcus aureus guide RNA

    Depositor

    Kiran Musunuru

    Insert

    Staphylococcus aureus guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-Fidgetin-C-18

    Plasmid

    #62756

    Purpose

    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Fidgetin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3-IL-2 signal_CyPet

    Plasmid

    #128029

    Purpose

    Mammalian expression vector with N terminal IL-2 signal peptide and CyPet

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    CyPet and IL-2 signal peptide

    Expression

    Mammalian

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-IL-2 signal_GST

    Plasmid

    #128032

    Purpose

    Mammalian expression vector with N terminal IL-2 signal peptide and GST

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    GST and IL-2 signal peptide

    Expression

    Mammalian

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEmerald-MyosinVIIA-C-14

    Plasmid

    #62762

    Purpose

    Localization: Cytoskeleton, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    MyosinVIIA
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. mEos2-MYR-N-5

    Plasmid

    #62768

    Purpose

    Localization: Cytoskeleton , N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581

    Depositor

    Michael Davidson

    Insert

    MYR
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits