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pCDNA3.1(+)-NEMOf

Plasmid

#189932

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOf

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOm

Plasmid

#189930

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOs

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin4A

Plasmid

#41914

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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p2092 HPV-18 E2

Plasmid

#10876

Depositor

Peter Howley

Article

Del Vecchio et al J Virol. 1992 Oct . 66(10):5949-58.

Insert

HPV18 E2 (E2 Human papillomavirus)

Expression

Mammalian

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Delta Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBBR15.2-2H12.D11opt-scarRef

Plasmid

#221150

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2.1
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen2.1)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-MBP-SCov2-nsp12

Plasmid

#154759

Purpose

Expression of SARS-CoV-2 nsp12

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Insect

Promoter

Polyhedron

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGW1-mCherry-EGFP-PIM

Plasmid

#111759

Purpose

High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

Depositor

Lukas Kapitein

Article

Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

Insert

2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

CMV

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX-PECAMTS

Plasmid

#45850

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

PECAM1 (PECAM1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 M337V

Plasmid

#27464

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-KG-CAND1

Plasmid

#19949

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pMIG FLAG B56 gamma3

Plasmid

#13803

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma 3 Flag (PPP2R5C Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_D17_hrGFP

Plasmid

#84616

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-AKT1-pcw107

Plasmid

#64606

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pNDamMyc

Plasmid

#59217

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Promoter

Drosophila heat-shock promoter

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBa.Kif5c 1-559.GFP

Plasmid

#45059

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif5c (Kif5c Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

Chicken Beta Actin

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only

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pTfR-mNeonGreen

Plasmid

#129608

Purpose

In vivo visualization of Transferrin receptors (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Transferrin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33A, S37A, T41A, S45A)-pcw107

Plasmid

#64612

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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