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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Bmf

Plasmid

#24257

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (BMF Human)

Tags

T7

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pYZ_3WJrep_N20_switch

Plasmid

#132722

Purpose

T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N20_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N20_trigger

Plasmid

#132726

Purpose

T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N20_trigger

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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SBP-EGFP-Ecadherin_puromycin

Plasmid

#65292

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Tensin-2 WT

Plasmid

#201797

Purpose

Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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1113 pSG5L HA PTEN D3

Plasmid

#10752

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

D3: S380D, T382D, T383D

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-proCaspase-3-TEVp

Plasmid

#166607

Purpose

Encodes Cre-dependent procaspase 3 and TEVp under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

procaspase3- TEVp

Use

AAV

Promoter

tetracycline response element

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-MT-mitfa

Plasmid

#81231

Purpose

expression of zebrafish mitfa

Depositor

James Lister, David Raible

Article

Lister et al Development. 1999 Sep;126(17):3757-67.

Insert

mitfa (mitfa Zebrafish)

Use

Zebrafish expression

Tags

6 myc tags

Expression

Mammalian

Mutation

contains amino acids 5-412

Promoter

sCMV IE94 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ2-Pdadh-LacZ

Plasmid

#191618

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP FAT

Plasmid

#50517

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase, domain

Tags

GFP

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pFLAG-ING2

Plasmid

#13295

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGSTag Bmf

Plasmid

#24264

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (BMF Human)

Tags

Gst

Expression

Bacterial

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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NpT7-5-Erk2

Plasmid

#39230

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

Erk2 (Mapk1 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pJK506

Plasmid

#155382

Purpose

dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus

Use

CRISPR

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-E2F-3'UTR-WT

Plasmid

#21170

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

E2F transcription factor 1 (E2F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-L10a

Plasmid

#166605

Purpose

Encodes Cre-dependent eGFP-L10a under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

eGFP-L10a

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N02_switch

Plasmid

#132742

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N02_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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