We narrowed to 10,323 results for: Coli
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Plasmid#66023PurposeMoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C]DepositorInsertRBS
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCD2_BC
Plasmid#66024PurposeMoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]DepositorInsertRBS
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCD8_BC
Plasmid#66025PurposeMoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C]DepositorInsertRBS
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0012m (lacI)_CD
Plasmid#66026PurposeMoClo Basic Part: CDS - Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010). Modified to fix illegal site. [C:C0012m:D]DepositorInsertTranscription factor
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0062 (luxR)_CD
Plasmid#66028PurposeMoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]DepositorInsertTranscription factor
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_EB
Plasmid#66005PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_FB
Plasmid#66006PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_GB
Plasmid#66007PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_AB
Plasmid#66008PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_EB
Plasmid#66009PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_FB
Plasmid#66010PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_GB
Plasmid#66011PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZS1[RbsR-T]
Plasmid#60751PurposeContains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.DepositorInsertRbsR-T
UseSynthetic BiologyExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…Available SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef-Psbtdh3-CSC-Tefm1
Plasmid#127742PurposeYeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.DepositorInsertcitrate synthase
UseSynthetic BiologyExpressionYeastPromoterPsbtdh3AvailabilityAcademic Institutions and Nonprofits only -
pOP3MT
Plasmid#112601PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusionsDepositorTypeEmpty backboneTagsAvi - 8His-MBPExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3GT
Plasmid#112607PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GST fusionsDepositorTypeEmpty backboneTagsAvi - 8His-GSTExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGM190oriT
Plasmid#115679PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertoriT, aphII, tsr
ExpressionBacterialPromotertipAAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOP2HA
Plasmid#112598PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusionsDepositorTypeEmpty backboneTags8His-Avi tagExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3BT
Plasmid#112603PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GB1 fusionsDepositorTypeEmpty backboneTagsAvi - 8His-GB1ExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3MP
Plasmid#112600PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and MBP fusionsDepositorTypeEmpty backboneTagsAvi - 8His-MBPExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3TP
Plasmid#112604PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and thioredoxin fusionsDepositorTypeEmpty backboneTagsAvi - 8His-thioredoxinExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3TT
Plasmid#112605PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and thioredoxin fusionsDepositorTypeEmpty backboneTagsAvi - 8His-thioredoxinExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3BP
Plasmid#112602PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GB1 fusionsDepositorTypeEmpty backboneTagsAvi - 8His-GB1ExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sc PCNA-pET(11a)
Plasmid#239197PurposeOverexpress Sc PCNA in E. coliDepositorInsertPCNA
ExpressionBacterialAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOP3GP
Plasmid#112606PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminal Avi-His8-tag and GST fusionsDepositorTypeEmpty backboneTagsAvi - 8His-GSTExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK BirA Hygro (w874-1)
Plasmid#29649DepositorInsertBirA
UseLentiviralExpressionMammalianAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-13XLexAop2-L4
Plasmid#41434DepositorInsert13XLexAop2
ExpressionBacterialAvailable SinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET32a-DEST
Plasmid#84652PurposeE. coli expression vector derived from pET32 with Gateway cassette.DepositorTypeEmpty backboneTagsHis, S tag, and Trx TagExpressionBacterialPromoterT7Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-13XLexAop2-R5
Plasmid#41433DepositorInsert13XLexAop2
ExpressionBacterialAvailable SinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
STK077_pSTK-0D-L3S2P21
Plasmid#235412PurposeVery strong terminator in B. subtilis. Strongest synthetic transcriptional terminator in E. coli. Recombination resistantDepositorInsertL3S2P21
ExpressionBacterialMutationNoAvailable SinceMarch 27, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1EDG
Plasmid#234144PurposeHeterologous protein expression of GUNA_RUMCH in Escherichia coliDepositorInsert1EDG
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
eforRed chromoprotein
Plasmid#117837PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coliDepositorInsertpromoter, RBS, eforRed
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS_375
Plasmid#182132Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMRDepositorInsertsretron RNA
ec.86 RT
CspRecT
Mutationincorporated rifampicin-resistance-conferring don…Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTM_COV2_NSP3_003_SUMO
Plasmid#233739PurposeExpresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease cleavage no nonnative residues remain on the PLpro proteinDepositorAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DNAseI
Plasmid#182409PurposeBacterial expression of Vesicle Nucleating peptide-DNaseI fusionDepositorInsertVNp-DNase I (DNASE1 Bovine)
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RNaseHI-NES-EGFP
Plasmid#196701PurposePlasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.DepositorInsertbacterial RNaseHI
UseLentiviralAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_AnapRS
Plasmid#174894PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertAnapRS
ExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)
Plasmid#85039PurposeFor AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.DepositorInsertRFP-P2A-tTA
UseAAVExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST_140_LVL2 cam
Plasmid#179334PurposeNT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).DepositorInsertPtac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRMutationCas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only