We narrowed to 3,380 results for: BID

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pAL189

Plasmid

#22278

Purpose

Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

Plasmid

#127360

Purpose

gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R

Plasmid

#104548

Purpose

Expresses Cry2-mCherry-PKA W196R in mammalian cells

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

Plasmid

#127357

Purpose

gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd (no NLS) SUP

Plasmid

#115490

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-AID*(N)

Plasmid

#99525

Purpose

N-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE671

Plasmid

#153237

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SIK1

Plasmid

#51577

Purpose

Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SIK1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(1-114)-8myc

Plasmid

#99516

Purpose

Truncated C-terminal AID-8myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

8xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SNF7

Plasmid

#51574

Purpose

Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SNF7 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-Rac1

Plasmid

#42955

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Mustard Weed, Human)

Use

Retroviral

Expression

Mammalian

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pEN-R2-mCsfGFP-L3

Plasmid

#178602

Purpose

It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

Depositor

Frederica Theodoulou

Article

Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

Insert

Tandem fluorescent protein timers (tFTs)

Use

Entry vector for gateway cloning

Tags

mCherry superfolder GFP

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

Plasmid

#112257

Purpose

Expresses RGS component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

CRY2-mCherry-(GGGGS)3-hRGS2box

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pLBM0090

Plasmid

#172824

Purpose

Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 3

Plasmid

#99318

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtUBQ10 (GB2478)

Plasmid

#160594

Purpose

UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtUBQ10

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE663

Plasmid

#153261

Purpose

M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE665

Plasmid

#153263

Purpose

M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE654

Plasmid

#153252

Purpose

M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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