We narrowed to 159,895 results for: addgene

1. pAG415Gal_Gcn4 StoL

   Plasmid

   #232981

   Purpose

   Galactose iduced expression of Gcn4 StoL in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 StoL
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S104L, S117L, S136L, S144L

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 R3W

   Plasmid

   #232975

   Purpose

   Galactose iduced expression of Gcn4 R++ W+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 R++ W+
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D103R, N126W, D133R, K143R

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG415Gal_Gcn4 WT 44mer

   Plasmid

   #232956

   Purpose

   Galactose iduced expression of Gcn4 WT 44mer in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 44mer
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG415Gal_Gcn4 E3HA

   Plasmid

   #232996

   Purpose

   Galactose iduced expression of Gcn4 E+HA in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 E+
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D105E, D118E, D139E

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG415Gal_Gcn4 WW+

   Plasmid

   #232984

   Purpose

   Galactose iduced expression of Gcn4 WW+ in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WW+
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   E109N, S117D, T121W, N126W, T132W

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG415Gal_Gcn4 F108A+

   Plasmid

   #232974

   Purpose

   Galactose iduced expression of Gcn4 F108A in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 F108A
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   F108A, T121L, A141P

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG415Gal_Gcn4 ILVtoAS

   Plasmid

   #232959

   Purpose

   Galactose iduced expression of Gcn4 ILVtoAS in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoAS
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   L123S, I128S, V130A, V135A, L137S, I142S

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG415Gal_Gcn4 WT 44merGFP

   Plasmid

   #233000

   Purpose

   Galactose iduced expression of Gcn4 WT 44merGFP in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 44mer
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG415Gal_Gcn4 WT 30mer

   Plasmid

   #232986

   Purpose

   Galactose iduced expression of Gcn4 WT 30mer in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 30mer
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV EGFP donor pDY0404

    Plasmid

    #219862

    Purpose

    AAV EGFP donor

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPB_EF1a_Gag-EIF4E-TM

    Plasmid

    #212658

    Purpose

    piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Piggybac

    Tags

    EIF4E (W56L, W102L, W166L)

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB_EF1a_Gag∆MA

    Plasmid

    #212654

    Purpose

    piggyBac vector to express MLV Gag with truncated MA domain

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of MA domain (amino acids 2-215)

    Promoter

    EF1a

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZB604_pT7-sfGFP

    Plasmid

    #228510

    Purpose

    Bacterial Hybrid Reporter Plasmid (sfGFP), pT7-sfGFP

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZB641_pT7_Lysin-KU1

    Plasmid

    #228511

    Purpose

    T7 RNAP inducible Lysin KU1 Expression

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    Lysin KU1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGFP-AUG-IRES-mCherry

    Plasmid

    #222109

    Purpose

    Start codon reporter (WT AUG)

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST39-Mega'-deltaAll

    Plasmid

    #216878

    Purpose

    Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but all gas vesicle proteins have been deleted.

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p8390 LentiCRISPRv2 Neo sgNT-2

    Plasmid

    #221650

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAC3-C-GFP-NLS

    Plasmid

    #222604

    Purpose

    Expression of MAC3-tagged GFP-NLS (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. MAC3-N-GFP-NES

    Plasmid

    #222606

    Purpose

    Expression of MAC3-tagged GFP-NES (N-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 3' nuclear exclusion sequence (NES)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. MAC3-NTRK3

    Plasmid

    #222612

    Purpose

    Expression of MAC3-tagged NTRK3

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    NTRK3 (NTRK3 Human)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge