We narrowed to 21,038 results for: cas9 genes

1. eSpCas9(1.1)-ABL1-gRNA1-exon4

   Plasmid

   #163322

   Purpose

   eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 1_Exon4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

2. eSpCas9(1.1)-ABL1-gRNA2-exon4

   Plasmid

   #163323

   Purpose

   eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

   Depositor

   Val Brunton

   Article

   Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

   Insert

   ABL1 gRNA 2_Exon4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

3. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

   Plasmid

   #165083

   Purpose

   gRNA 2 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

   Plasmid

   #165085

   Purpose

   gRNA 1 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-TREX1 g3 Cas9-T2A-mCherry

   Plasmid

   #164252

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 nickase D10A Cas9 (GB1691)

   Plasmid

   #160588

   Purpose

   Nickase D10A Cas9 for Nt fusion.

   Depositor

   Diego Orzaez

   Insert

   nickase D10A Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

   Plasmid

   #149346

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbc

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

   Plasmid

   #149347

   Purpose

   Mammalian expression vector for SpyCas9 and puromycin resistance

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

    Plasmid

    #159919

    Purpose

    Mutagenesis of Vamp1

    Depositor

    Larry Zweifel

    Insert

    Vamp1 (Vamp1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid

    #159907

    Purpose

    Mutagenesis of Netrin1

    Depositor

    Larry Zweifel

    Insert

    Netrin1 (Ntn1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

    Plasmid

    #159906

    Purpose

    Mutagenesis of Dcc

    Depositor

    Larry Zweifel

    Insert

    Dcc (Dcc Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid

    #132927

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Insert

    humanized SpCas9
    

    Use

    CRISPR and Synthetic Biology; Pmagic gateway entr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

    Plasmid

    #121827

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-Cas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. A3H HapII R175/6E-Cas9n-UGI

    Plasmid

    #119142

    Purpose

    Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A3H Hap II RR175/6EE

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459V2.0-eSpCas9(1.1)+K810A+K855A

    Plasmid

    #108299

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Px330-EGFP-LRRK2-CRISPR/Cas9

    Plasmid

    #180437

    Purpose

    Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    gRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK-caCas9-NatMX-Neut5L-Ade2 drive

    Plasmid

    #89577

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Ade2 gene drive
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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