-
-
pTRE3G-human cGAS-HA
Plasmid#186842PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS with C-terminal HA tag (Adamts1 Human)
UseRetroviralTagsHAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl2-Cb5
Plasmid#18000DepositorInsertER targeted Bcl-2 (BCL2 Human)
UseTagsGFPExpressionMammalianMutationBcl-2's C terminal targeting sequence replac…PromoterCMVAvailable sinceJune 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
UseTagsExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_M18
Plasmid#82356PurposeExpression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-FLPo-Myc
Plasmid#124641PurposeAAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoterDepositorInsertFLPo
UseAAV and Cre/LoxTagsmyc tagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcGFP-MLH1
Plasmid#215120PurposeExpresses AcGFP-MLH1 in mammalian cells from a retroviral vector.DepositorInsertMLH1 (MLH1 Human)
UseRetroviralTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM_M18
Plasmid#91729PurposeExpression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
UseTagsExpressionMammalianMutationPromoterhEF1-HTLV promAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-Bcl2-Cb5
Plasmid#18004DepositorInsertER targeted Bcl-2 (BCL2 Human)
UseTagsFlagExpressionMammalianMutationC terminal of Bcl-2 replaced with Cytochrome b5. …PromoterCMVAvailable sinceJune 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-EF1a-FDIO-ArchT-GFP
Plasmid#124640PurposeAAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoterDepositorInsertArchT
UseAAV; Flp/frtTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-αIFNα-ab
Plasmid#110799PurposeExpression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cellsDepositorInsertantibody against mouse interferon alpha
UseTagsER signal peptide, His6 tag, and myc epitope tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP mScarlet-MOSPD2 W201E
Plasmid#186477PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsmScarletExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTER RB1shRNA human
Plasmid#66886PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against RB1DepositorInsertretinoblastoma 1 (RB1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PNKP
Plasmid#215137PurposeExpresses mCherry-PNKP in mammalian cells from a retroviral vector.DepositorInsertPNKP (PNKP Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL
Plasmid#63885PurposeAn AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.DepositorInsertER-localized low-affinity GCaMP3(10.19)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176459PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA
Plasmid#176461PurposeVector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMVDepositorInsertBbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(deltaNIC)
Plasmid#110191PurposeExpression of mouse MeCP2 (deltaNIC) in mammalian cellsDepositorInsertMeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationdelta N and C termini. Intervening region replace…PromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tbg-CES2A-ΔC-S227A
Plasmid#200560PurposeSecreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vectorDepositorInsertMouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
UseAAVTagsFlagExpressionMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only
