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We narrowed to 6,891 results for: GFP expression plasmids

Showing: 6301 - 6350 of 6891 results

  16. pCRISPR-S12

    Plasmid
    #84031
    Purpose
    To episomally express codon optimized Cas9 and chimeric guide RNA
    Depositor
    Sen Wu
    Article
    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:
    Inserts
    hSpCas9
    eGFP
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and CMV (downstream of F2A self-cleaving pept…
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    84031-plasmid-map-sequence-id-192362

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  27. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    7xTCF-LEF and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  28. pIFEX-A2aGH

    Plasmid
    #160542
    Purpose
    pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160542-plasmid-map-sequence-id-317791

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  29. pEFEX1-A2aGH

    Plasmid
    #160543
    Purpose
    pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160543-plasmid-map-sequence-id-317760

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  30. pCFEX1-A2aGH

    Plasmid
    #160546
    Purpose
    pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    N/A
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160546-plasmid-map-sequence-id-317845

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  34. p458 VRER

    Plasmid
    #101728
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Article
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Insert
    SpCas9 VRER
    Use
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Promoter
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    101728-plasmid-map-sequence-id-196591

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  35. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112861-plasmid-map-sequence-id-296442

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  39. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  41. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  43. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

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  45. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid
    #239102
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Article
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    POU4F2 (POU4F2 Human)
    Use
    Donor plasmid for integration into the human geno…
    Tags
    p2A-tdTomato-p2A-Thy1.2
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous POU4F2
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239102-plasmid-map-sequence-id-474475

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Showing: 6301 - 6350 of 6891 results