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CD8a-AA358-361-GFP

Plasmid

#247767

Purpose

Expresses CD8a fused with a segment of the Arl13b protein (amino acids 358-361) and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 354-361 are inserted

Promoter

CMV promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4b)

Plasmid

#241044

Purpose

Expresses SARS-CoV-2 spike (B.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,K529L,D614G,R683L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4c)

Plasmid

#241045

Purpose

Expresses SARS-CoV-2 spike (B.1-4c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N439R,T478K,E484V,D614G,K529L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4a)

Plasmid

#241042

Purpose

Expresses SARS-CoV-2 spike (B.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,F490R,D614G,R683N

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4d)

Plasmid

#241043

Purpose

Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2c)

Plasmid

#241040

Purpose

Expresses SARS-CoV-2 spike (B.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2d)

Plasmid

#241041

Purpose

Expresses SARS-CoV-2 spike (B.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-TSC22D1-Brd 140

Plasmid

#219104

Purpose

Transcription factor TSC22D1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

TSC22D1 (TSC22D1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VSIG2-COMP5AP-AviTag-9xHis

Plasmid

#157223

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSIG2 (VSIG2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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MAST3 gRNA (BRDN0001147549)

Plasmid

#76104

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAST3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAST3 (MAST3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-ATAD2-plx303

Plasmid

#188635

Purpose

ATAD2 overexpression

Depositor

Lorenz Studer

Article

Baggiolini et al Science. 2021 Sep 3;373(6559):eabc1048.

Insert

ATAD2 (ATAD2 Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pX458M-53BP1-DN1S

Plasmid

#131045

Purpose

Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

Depositor

Punam Malik

Article

Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

Insert

SpCas9-53BP1-DN1S (TP53BP1 Human)

Use

CRISPR

Tags

GFP and SpCas9

Expression

Mammalian

Mutation

Only expressing residues 1231-1644 of complete 53…

Promoter

CBH

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB3-V5/HIS

Plasmid

#201104

Purpose

expression of human ERBB3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human ERBB3 receptor tyrosine kinase, full length, wildtype (ERBB3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-HtrA-ss

Plasmid

#137066

Purpose

E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66500.2 (BB_0104 Borrelia burgdorferi B31)

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature HtrA: lacks the HtrA signal peptide

Promoter

T7lac

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A12_STOP

Plasmid

#161104

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A12 (SLC25A12 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC16A9_STOP

Plasmid

#161042

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A9 (SLC16A9 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC1A1_STOP

Plasmid

#161044

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC1A1 (SLC1A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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