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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,213 results for: GCA

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Showing: 2521 - 2540 of 8213 results

  2. pFA0055

    Plasmid
    #131774
    Purpose
    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131774-plasmid-map-sequence-id-258731

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  8. pQCascade-IS2

    Plasmid
    #140625
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140625-plasmid-map-sequence-id-305996

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  12. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid
    #173214
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173214-plasmid-map-sequence-id-339507

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  15. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  20. pCY5.1kh

    Plasmid
    #160297
    Purpose
    Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    160297-plasmid-map-sequence-id-484567

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Showing: 2521 - 2540 of 8213 results