We narrowed to 173,477 results for: addgene

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PNCA-GFP

Plasmid

#67789

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

Insert

MMLV-LTR-GFP

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pBI-MCS-EYFP

Plasmid

#58855

Purpose

The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pNH-NanoLuc

Plasmid

#173075

Purpose

Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-mRFP

Plasmid

#36972

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pHCMV_DUGV_GP

Plasmid

#207268

Purpose

Mammalian expression of Dugbe virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DUGV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2plus

Plasmid

#164226

Purpose

Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

Depositor

Silvia Cardona

Article

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Expression

Bacterial

Promoter

Rhamnose-inducible

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-ROSA26_hspCas9

Plasmid

#193310

Purpose

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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LacZ_pHIVEGFP

Plasmid

#191251

Purpose

Expresses LacZ in mammalian cells (used as a control)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LacZ

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNM3689

Plasmid

#154111

Purpose

miniMos single component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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ERoxBFP

Plasmid

#68126

Purpose

mammalian expression of ER localized oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

oxBFP

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
ATG16L1 sgRNA

Plasmid

#207563

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

GCAGCAAGTGACATGTCGTC

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pNup133-Ypet

Plasmid

#98283

Purpose

mammalian expression of Nup133-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Nup133 (NUP133 Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHCMV_LASV_GPC

Plasmid

#207287

Purpose

Mammalian expression of Lassa virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

LASV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pACUH

Plasmid

#58374

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han, Yuh-Nung Jan

Article

Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

Type

Empty backbone

Expression

Insect

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pL1P4 ZmUbiP:GRF-GIF:NosT

Plasmid

#198048

Purpose

MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

Depositor

Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

Insert

ZmUbiP::TaGRF4-GIF1::NosT

Use

Synthetic Biology

Promoter

Zea mays (maize) ubiquitin promoter

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_FLUCV_HE

Plasmid

#207275

Purpose

Mammalian expression of Influenza C virus HE protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUCV_HE

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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