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We narrowed to 6,891 results for: GFP expression plasmids

Showing: 6351 - 6400 of 6891 results

  18. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  25. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Article
    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  26. LPUtopia-7

    Plasmid
    #199212
    Purpose
    Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Inserts
    aminoglycoside phosphotransferase,
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and thymidine kinase promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199212-plasmid-map-sequence-id-396462

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  27. pAAV-UBC-S1-GCaMP6f

    Plasmid
    #160722
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160722-plasmid-map-sequence-id-315521

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  28. pAAV-UBC-S2-cAMPr

    Plasmid
    #160723
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S2-cAMPr
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160723-plasmid-map-sequence-id-315524

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  29. pAAV-UBC-S3-ExRaiAKAR

    Plasmid
    #160724
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-ExRaiAKAR
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160724-plasmid-map-sequence-id-315754

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  30. pAAV-UBC-S4-ExRaiCKAR

    Plasmid
    #160725
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S4-ExRaiCKAR
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160725-plasmid-map-sequence-id-315528

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  31. pLenti-CMV-human cGASdeltaN-OMM

    Plasmid
    #186887
    Purpose
    Expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain fusion (Adamts1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    CMV
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186887-plasmid-map-sequence-id-366443

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  32. pLenti-CMV-orangutan cGASdeltaN-OMM

    Plasmid
    #186888
    Purpose
    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal MAVS transmembrane domain fusion (CGAS Synthetic, Pongo abelii)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    CMV
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186888-plasmid-map-sequence-id-366446

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  33. pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM

    Plasmid
    #186889
    Purpose
    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with N-terminal FLAG tag and C-terminal MAVS transmembrane domain fusion (CGAS Synthetic, Pongo abelii)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    CMV
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186889-plasmid-map-sequence-id-366449

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  34. pLV-SFFV-CALU-WPRE-UbC-Emerald

    Plasmid
    #225951
    Purpose
    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Bart Nieuwenhuis, Richard Eva
    Inserts
    CALU (CALU Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225951-plasmid-map-sequence-id-446853

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  35. pLV-SFFV-LNPK-WPRE-UbC-Emerald

    Plasmid
    #225953
    Purpose
    Lentiviral vector plasmid expressing human lunapark (LNPK) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Evan Reid, Bart Nieuwenhuis, Richard Eva
    Inserts
    LNPK (LNPK Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225953-plasmid-map-sequence-id-446858

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  36. pYN2_1

    Plasmid
    #184757
    Purpose
    Plasmid for genome editing by CRISPR/Cas9
    Depositor
    Yvonne Nygård
    Article
    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10
    Inserts
    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    Use
    Synthetic Biology
    Tags
    2xNLS
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    PGK1 and tRNA-Phe-HDV Ribozyme
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    184757-plasmid-map-sequence-id-421882

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  39. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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  43. Artichoke

    Plasmid
    #73320
    Purpose
    Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    73320-plasmid-map-sequence-id-135028

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  45. AAV-Dlx-SynaptoTAG

    Plasmid
    #210729
    Purpose
    AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.
    Depositor
    Wei Xu
    Article
    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050
    Insert
    EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Synthetic, Rat)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Dlx
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    210729-plasmid-map-sequence-id-418041

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  48. p2attNG-H11-short

    Plasmid
    #51546
    Purpose
    p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    GFP4m
    Use
    Human targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51546-plasmid-map-sequence-id-78774

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  49. AAV-∆Hapln1

    Plasmid
    #200778
    Purpose
    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter
    Depositor
    Alexander Dityatev
    Article
    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d
    Insert
    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    Use
    AAV
    Tags
    cfGFP
    Expression
    Mutation
    deleted lectican binding domain (aminoacids 40-15…
    Promoter
    Synapsin
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200778-plasmid-map-sequence-id-394043

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Showing: 6351 - 6400 of 6891 results