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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,242 results for: grna

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Showing: 2541 - 2560 of 16242 results

  11. RUBY-ABE#2

    Plasmid
    #232176
    Purpose
    T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.
    Depositor
    Yiping Qi
    Publication
    Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265
    Insert
    2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold
    Use
    CRISPR
    Tags
    3xflag and NLS
    Expression
    Plant
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    232176-plasmid-map-sequence-id-459941

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  12. pYPQAT58

    Plasmid
    #223430
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223430-plasmid-map-sequence-id-454181

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  13. pYPQAT13

    Plasmid
    #223385
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223385-plasmid-map-sequence-id-453298

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  14. dead Sa Cas9

    Plasmid
    #138162
    Purpose
    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSaCas9
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D10A + N580A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138162-plasmid-map-sequence-id-272344

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  15. AV13_pCAG.Cas9.gRNA.NT

    Plasmid
    #199260
    Purpose
    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199260-plasmid-map-sequence-id-391374

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Showing: 2541 - 2560 of 16242 results