We narrowed to 6,334 results for: nls

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pCYB-TnsA

Plasmid

#17356

Depositor

Nancy Craig

Article

Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

Insert

TnsA (tnsA E. coli transposon Tn7)

Tags

Intein-Chitin binding domain

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCMV5 MEKK2 KD

Plasmid

#12183

Depositor

Gary Johnson

Article

Fanger et al EMBO J. 1997 Aug 15. 16(16):4961-72.

Insert

MEKK2 (Map3k2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K385M

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
LEXY TR (without KRAB domain) (pDN162)

Plasmid

#72661

Purpose

LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

LexATF-T2A-NLS-LexA-mCherry-LEXY

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mGFP-WDR34

Plasmid

#64251

Purpose

Expresses WDR34 as a fusion with monomeric GFP

Depositor

David Stephens

Article

Asante et al J Cell Sci. 2014 Nov 1;127(21):4774-87.

Insert

WDR34 (WDR34 Human)

Use

Lentiviral

Tags

mGFP

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
LEXY TR (with KRAB domain) (pDN163)

Plasmid

#72662

Purpose

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pKM065

Plasmid

#134175

Purpose

Retroviral plasmid expressing GFP-NLS-Plk1-PBD

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

GFP-NLS-Plk1-PBD 326-603 (PLK1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

PBD 326-603

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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px330 EQR

Plasmid

#101733

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBC2102-reci

Plasmid

#117171

Purpose

U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_wtCas9 without NLS

Use

Lentiviral

Promoter

U6 Promoter

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mRuby-WDR34

Plasmid

#66599

Purpose

Expresses WDR34 as a fusion with monomeric mRuby

Depositor

David Stephens

Article

Asante et al J Cell Sci. 2014 Nov 1;127(21):4774-87.

Insert

WDR34 (WDR34 Human)

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
Sus1/PET32a

Plasmid

#26963

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sus1 (SUS1 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCHW00015

Plasmid

#222446

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

VP64-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00014

Plasmid

#222445

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

VP16-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV/CMV-SCLM

Plasmid

#216758

Purpose

AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

Depositor

George Augustine

Insert

SuperClomeleon followed by WPRE and human growth hormone polyA terminator

Use

AAV

Expression

Mammalian

Mutation

S30R and 11 AA deletion in the Cerulean CFP moiet…

Promoter

CMV (cytomegalovirus)

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ300

Plasmid

#205414

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

Depositor

Yiping Qi

Article

Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

Insert

Ev1Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS (N terminal on insert) NLS (C terminal on ins…

Expression

Plant

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3477

Plasmid

#190416

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XPhlFO-3XAmtRO::TetR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

6XPhlFO-3XAmtRO::TetR-NLS-19St

Expression

Plant

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3617

Plasmid

#190427

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (pro35S::TetR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

pro35S::TetR-NLS-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3017

Plasmid

#190399

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::McbR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::McbR-NLS-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2964

Plasmid

#190398

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XAmtRO-2XPhlFO::TetR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

6XAmtRO-2XPhlFO::TetR-NLS-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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EC0_3_Mxi1

Plasmid

#163715

Purpose

Plasmid encoding Mxi1 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

Mxi1+SV40 NLS

Use

Synthetic Biology; Moclo vector level 0 (type 4a)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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