We narrowed to 3,461 results for: mpo

1. pMXS-IRES-Blast POLD1

   Plasmid

   #160805

   Purpose

   Express POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized C-terminal 230 amino acids

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. phBG-P53DD

   Plasmid

   #149707

   Purpose

   In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

   Depositor

   Erika Sasaki

   Article

   Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   Rna transcription

   Tags

   Expression

   Mutation

   deleted amino acid 14-301

   Promoter

   T7

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-OptoFGFR1-Y766F

   Plasmid

   #59777

   Purpose

   Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

   Depositor

   Won Do Heo

   Article

   Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

   Insert

   OptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Hyg-Snrnp40-CRISPR-resistant

   Plasmid

   #134251

   Purpose

   Lentivector encoding CRISPR-resistant Snrnp40

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mutated coding sequence “gataactatgcgacgttgaa” to…

   Promoter

   EF1a

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

   Plasmid

   #169166

   Purpose

   Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

   Insert

   3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3xFLAG-CMV-Drosha (NLS-P426)

   Plasmid

   #171538

   Purpose

   Encodes mutant human Drosha protein

   Depositor

   Yan Zeng

   Article

   Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

   Insert

   DROSHA (DROSHA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   an SV40 NLS sequence (PKKKRKVGI) insertion into t…

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. J7AAV-HDR Fah.2

   Plasmid

   #73451

   Purpose

   J7AAV-HDR U6sgFah.2 template2 to repair Fah mutation

   Depositor

   Wen Xue

   Article

   Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

   Insert

   Fah (Fah Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-3xNLS-OptoJNKi-dsm

   Plasmid

   #89743

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi dark-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-3xNLS-OptoJNKi-lsm

   Plasmid

   #89742

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi lit-state mutant
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   I539E mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FKBP-beta2(616-951)-GFP Double

    Plasmid

    #100729

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif and …

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. J8AAV-HDR Ctnnb1.1

    Plasmid

    #73452

    Purpose

    J8AAV-HDR U6sgCtnnb1.1 template

    Depositor

    Wen Xue

    Article

    Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

12. FKBP-beta2(616-951)-GFP ∆CBM

    Plasmid

    #100728

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif

    Promoter

    CMV

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/NDUFS2 _34-78/myc-His

    Plasmid

    #127496

    Purpose

    Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _34-78 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _34-78

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. FKBP-beta2(616-951)-GFP Y215A

    Plasmid

    #100727

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform Y215A mutation (Y815A in full length)

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAED2

    Plasmid

    #234599

    Purpose

    Fluorescent reporter of the SOS response

    Depositor

    Tanel Tenson

    Article

    Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):

    Inserts

    mScarlet-I
    gfp-mut2
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    GFPmut2 was derived from avGFP with the following…

    Promoter

    Ptet+dnaK P1 and cda

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. NST3_pOpOn2.1

    Plasmid

    #228066

    Purpose

    Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

    Depositor

    Charles Anderson

    Article

    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

    Insert

    NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)
    

    Use

    Dexamethasone inducible vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNK7190

    Plasmid

    #219763

    Purpose

    MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    nnLuz, nnH3H, nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcs2-cDELE1(142-515)-sumo

    Plasmid

    #226107

    Purpose

    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    DELE1 (DELE1 Human)
    

    Use

    Tags

    Sumo

    Expression

    Mammalian

    Mutation

    encodes only residues 142-515 of DELE1 (represent…

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP-GFP-TRPML1(PHD)

    Plasmid

    #221752

    Purpose

    Stable expression of GFP-TRPML1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TRPML1(PHD) (amino acids 1–69)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CCM2(413-438)_pHisSUMO(K0)

    Plasmid

    #224065

    Purpose

    Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site

    Depositor

    Ben Goult

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    ccm2 (CCM2 Human)
    

    Use

    Tags

    6xHIS; SUMO(K0)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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