We narrowed to 5,763 results for: org

1. pYPQ269A2

   Plasmid

   #164714

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
   

   Use

   CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

   Plasmid

   #109318

   Purpose

   Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   SaCas9 RuvC gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATP1A1_G8_Dual_sgRNA

   Plasmid

   #178104

   Purpose

   Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265

   Plasmid

   #164712

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   rAPOBEC1-zCas9(D10A)-UGI
   

   Use

   CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tdminiSOG-H2B-C-10

   Plasmid

   #57804

   Purpose

   Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   tdminiSOG

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pST_SERF2_HUMAN (pBS0751)

   Plasmid

   #185239

   Purpose

   For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   SERF2_HUMAN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pST_MAHS_RAMVA (pBS0310)

   Plasmid

   #185169

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKI-cjFOXL2-coemiRFP670-PGK-Puro

   Plasmid

   #186168

   Purpose

   Knock-in gene targeting in marmoset cells at FOXL2 locus

   Depositor

   Sho Yoshimatsu

   Article

   Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

   Insert

   emiRFP670
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniSOG-Tubulin-6

   Plasmid

   #57777

   Purpose

   Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pYPQ269D

    Plasmid

    #164717

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v04 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

    Plasmid

    #115139

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA3.1 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. kiCAP-AAV-M.Mus.1

    Plasmid

    #196681

    Purpose

    Rep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WVLPSGG insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. kiCAP-AAV-MDV1B

    Plasmid

    #196680

    Purpose

    Rep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    KTVGTVY insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pminiT-Halo-MED1 donor

    Plasmid

    #197868

    Purpose

    Donor plasmid used for building Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Halotag

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST_DHN_AtCor47 (pBS0784)

    Plasmid

    #185254

    Purpose

    For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHN_AtCor47
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

    Plasmid

    #127787

    Purpose

    Expresses a SsCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    mutations in His29, Arg32, Arg36 and Arg69 to Ala

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b-ITPA

    Plasmid

    #60824

    Purpose

    to purify human ITPA from bacteria

    Depositor

    Youri Pavlov

    Article

    Porta et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    ITPA (ITPA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC94

    Plasmid

    #118912

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease and eGFP-HIS6

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid

    #115140

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA3 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid

    #115138

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge