We narrowed to 8,708 results for: sgRNA

1. pLenti-AncBE4max

   Plasmid

   #174164

   Purpose

   This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

   Depositor

   Wensheng Wei

   Article

   Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

   Insert

   AncBE4max
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKRG3-mU6-PUb-hSpCas9-pAc

   Plasmid

   #162163

   Purpose

   Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   T2A-pAc

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR v2-sgMyc_1

   Plasmid

   #138324

   Purpose

   Express guide RNA 2 for mouse Myc

   Depositor

   Yi Zhang

   Article

   Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

   Insert

   cMyc sgRNA (Myc Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-sgMyc_2

   Plasmid

   #138346

   Purpose

   Express guide RNA 1 for mouse Myc

   Depositor

   Yi Zhang

   Article

   Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

   Insert

   cMyc sgRNA (Myc Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP gRNA (BRDN0000563266)

   Plasmid

   #80036

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKRG3-mU6-PUb-3xFLAG-hSpCas9

   Plasmid

   #162161

   Purpose

   Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-cBEST-v2-PermE

   Plasmid

   #209446

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from ermE* promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N

   Plasmid

   #164907

   Purpose

   Express a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2

   Depositor

   Wen Xue

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   pegSERPINA1, Nicking sgRNA, PE2-N
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-CANX

   Plasmid

   #227279

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CANX (CANX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL-CRISPR.SFFV.tRFP

    Plasmid

    #57826

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-CLTC

    Plasmid

    #227312

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CLTC (CLTC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP gRNA (BRDN0000561167)

    Plasmid

    #80034

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-PEX3

    Plasmid

    #227303

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-RAB7A

    Plasmid

    #227297

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of RAB7A (RAB7A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid

    #178091

    Purpose

    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster U6:3

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP gRNA (BRDN0000562805)

    Plasmid

    #80035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid

    #178090

    Purpose

    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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