Skip to main content
Addgene
Showing: 481 - 520 of 18068 results

  1. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH2 (MDH2 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204230-plasmid-map-sequence-id-413333

    Enlarge

  8. C-SWAT GFP CL1 DAmP

    Plasmid
    #182513
    Purpose
    C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    CL1 and DAmP
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182513-plasmid-map-sequence-id-358273

    Enlarge

  11. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Use
    Tags
    Met-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

    Enlarge

  14. SG3ΔENV CA M66I

    Plasmid
    #149687
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).
    Depositor
    Wesley Sundquist
    Article
    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/
    Insert
    Capsid
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Capsid residue Met 66 to Iso (CA M66I)
    Promoter
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149687-plasmid-map-sequence-id-304544

    Enlarge

  16. pGCNK58

    Plasmid
    #202461
    Purpose
    Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells
    Depositor
    Stephen Harrison, Arthur Palmer
    Article
    Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi
    Insert
    Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    202461-plasmid-map-sequence-id-403118

    Enlarge

  21. CMV/N3IC-HA

    Plasmid
    #47618
    Purpose
    in vitro transcription and translation of HA-tagged Notch3 Intracellular domain
    Depositor
    Urban Lendahl
    Article
    Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.
    Insert
    Notch3 Intracellular domain (Notch3 Mouse)
    Use
    In vitro transcription and translation
    Tags
    HA
    Expression
    Mammalian
    Mutation
    codons 1664 (Met) to 2318 (Ala)
    Promoter
    CMV
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. Rtn2b-RHD272-472-GFP

    Plasmid
    #186606
    Purpose
    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFP
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Met-Gly-Ser-Lys peptide added at N-terminus for c…
    Promoter
    CMV
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186606-plasmid-map-sequence-id-369199

    Enlarge

  35. Rtn2b-RHD272-472-mNeon

    Plasmid
    #186605
    Purpose
    Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen
    Depositor
    Gia Voeltz
    Article
    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6
    Insert
    Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Met-Gly-Ser-Lys peptide added at N-terminus for c…
    Promoter
    CMV
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186605-plasmid-map-sequence-id-369207

    Enlarge

  38. Ma-PylRS-lib1

    Pooled Library
    #224585
    Purpose
    Pyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Expression
    Bacterial
    Species
    Methanomethylophilus alvus
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only

  40. pDCAF3-IS5

    Plasmid
    #65220
    Purpose
    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.
    Depositor
    Jeffrey Barrick
    Article
    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.
    Inserts
    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed start codon from gtg to atg and Changed s…
    Promoter
    BBa_J23100
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    65220-plasmid-map-sequence-id-134450

    Enlarge

Showing: 481 - 520 of 18068 results