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pET23-CBD-(-PP)-EGFP
Plasmid#12597DepositorInsertCdc42 Biosensor (WAS Human)
UseTags6xHis and EGFPExpressionBacterialMutationF271CPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
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NTM (FLM-PC1Δ137)
Plasmid#41572DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationsequence of PKD1 FLM from 4078 - 4273PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ30
Plasmid#41568DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ30; sequence of PKD1 from 4078 - 4273PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ45
Plasmid#41569DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ45; sequence of PKD1 from 4078 - 4258PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ67
Plasmid#41570DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ67; sequence of PKD1 from 4078 - 4236PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLM-PC1Δ103
Plasmid#41571DepositorInsertPKD1 (PKD1 Human)
UseTagsCD16.7ExpressionMammalianMutationΔ103; sequence of PKD1 from 4078 - 4200PromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
BSA2_His
Plasmid#202767Purposebovine serum albumin design BSA2, in pET29b with C-terminal 6His-tagDepositorInsertBSA2
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
BSA3_His
Plasmid#202768Purposebovine serum albumin design BSA3, in pET29b with C-terminal 6His-tagDepositorInsertBSA1
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; T3_gRNA
Plasmid#199335PurposepDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNADepositorInsertszCREST3
Cas9
nrg1 type III sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-pknG
Plasmid#102814PurposeExpression of PknG in E.coliDepositorInsertpknG
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
68Q-ymsfGFP
Plasmid#128560PurposeHttex1 (without proline-rich region) fused to ymsfGFPDepositorInsert68Q-Httex1 (without proline-rich region) fused to ymsfGFP
UseTagsymsfGFPExpressionYeastMutationPromoterGAL1Available sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mpeg:lamp2-mCherry-stop; cmlc2:GFP
Plasmid#213740PurposeExpresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis markerDepositorInsertlamp2-mCherry
UseZebrafishTagsExpressionMutationPromotermpeg1.1Available sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
mpeg:Rab5c-stop-polyA; cmlc2:GFP
Plasmid#213737PurposeExpresses mCherry-Rab5c in the macrophage lineage of zebrafish with a green heart transgenesis markerDepositorInsertmCherry-Rab5c
UseZebrafishTagsmCherryExpressionMutationPromotermpeg1.1Available sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T3_gRNA
Plasmid#199389PurposepDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNADepositorInsertnrg1 type III sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA010.188
Plasmid#206792PurposeJ23113.dCas9DepositorInsertdCas9
UseTagsExpressionMutationPromoterJ23113Available sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK389
Plasmid#206791PurposeSp.dCas9, pTet.MCP-SoxS, J23119.gRNADepositorInsertsdCas9
MCP-SoxS
scRNA
UseTagsExpressionMutationPromoterJ23119 and TetAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT009.119.DA9
Plasmid#206803PurposeExpresses DA9 scRNA for activation of pDA303DepositorInsertDA9 scRNA
UseTagsExpressionMutationPromoterJ23119Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; T2_gRNA
Plasmid#197647PurposepDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNADepositorInsertszCREST3
Cas9
nrg1 type II sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; T1_gRNA
Plasmid#199332PurposepDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNADepositorInsertszCREST3
Cas9
nrg1 type I sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
25Q-ymsfGFP
Plasmid#128559PurposeHttex1 (without proline-rich region) fused to ymsfGFPDepositorInsert25Q-Httex1 (without proline-rich region) fused to ymsfGFP
UseTagsymsfGFPExpressionYeastMutationPromoterGal1Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
68Q-ymScarlet
Plasmid#128562PurposeHttex1 (without proline-rich region) fused to ymScarletDepositorInsert68Q-Httex1 (without proline-rich region) fused to ymSarlet
UseTagsymScarletExpressionYeastMutationPromoterGal1Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
25Q-ymScarlet
Plasmid#128561PurposeHttex1 (without proline-rich region) fused to ymScarletDepositorInsert25Q-Httex1 (without proline-rich region) fused to ymSarlet
UseTagsymScarletExpressionYeastMutationPromoterGAL1Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD06 K. lactis telomerase RNA hairpin
Plasmid#61895PurposePlasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)DepositorInsertTelomerase RNA hairpin (nt 229-273)
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42(wt)
Plasmid#12599DepositorInsertEGFP-Cdc42 (WT) (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET11a-4SS-HEWL
Plasmid#39233DepositorInsertHen egg white lysozyme (LYZ Chicken)
UseTagsExpressionBacterialMutationN-terminal MetPromoterT7Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
MCP/PCP RNA Lantern
Plasmid#226095PurposeRNA trackingDepositorInsertNLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEFmEGFP-mDia2
Plasmid#25407DepositorInsertmDia2 (Diaph3 Mouse)
UseTagsEGFP, His, and MycExpressionMammalianMutationEncodes mDia2PromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PV-NES-GFP
Plasmid#17301DepositorInsertPV-NES-GFP (Pvalb Rat)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS156
Plasmid#182707PurposeCRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VP192-T2A-EGFP-shP53
Plasmid#69535PurposeEpisomal plasmid encoding dCas9VP192 and p53 shRNADepositorInsertdCas9-dCas9VP192-GFP-shp53
UseCRISPRTagsEGFPExpressionMutationPromoterCAGAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pADH100
Plasmid#90980PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUGW-UBC-EGFP-2A-BoNT-B (LC)
Plasmid#185722PurposeLentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoterDepositorInsertbotulinum neurotoxin type B light chain
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OCT4
Plasmid#69537PurposeEpisomal plasmid encoding 5 gRNAS targeting human OCT4 promoterDepositorInsert5 concatenated gRNA transcriptional cassettes targeting OCT4
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-mDia1FH2deltaN
Plasmid#25419DepositorInsertdiaph1 (Diaph1 Mouse)
UseTagsYFP A206KExpressionMammalianMutationmDia1 FH2∆N2 sequence (encodes amino acids 771-11…PromoterAvailable sinceJuly 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRRG389-PC2
Plasmid#99087PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea prohormone convertase 2 (PC2)DepositorInsertprohormone convertase 2 (PC2)
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRG757-smedwi-1
Plasmid#99074PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Smedwi-1DepositorInsertSmedwi-1 in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-Z3EV
Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)TagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sport2-mGCN5 FU, FLAG Tagged
Plasmid#23098DepositorInsertmouse Gcn5 mRNA (Kat2a Mouse)
UseTagsFLAGExpressionMammalianMutationmissing 50 bp upstream of ATG start site.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pADH147
Plasmid#90991PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.DepositorInsertgRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAR-mOrange2-sfGFP
Plasmid#115693PurposeGeneration of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent TimerDepositorInsertSA-mOrange2-sfGFP-SD
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd-PV-NLS-CD-DsRed
Plasmid#16345DepositorInsertPV-DsRed-NLS-CD (Pvalb Rat)
UseAdenoviralTagsMycExpressionMammalianMutationFunctional Ca2+binding site CD was inactivated by…PromoterAvailable sinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto roGFP2-Orp1
Plasmid#64996Purposefor expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCaSper4 cyto roGFP2-Orp1
Plasmid#64998PurposeExpression of cytosolic roGFP2-Orp1 under the alphaTub84B promoterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2_(-3kb)nrg1_typeII:GFP-CAAX
Plasmid#199394PurposepDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region controlDepositorInserts(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR
UseTol2 gateway destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRG203-notum
Plasmid#99067PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea notumDepositorInsertnotum in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plas-CRISPR_reporter
Plasmid#157658PurposeCRISPR reporter for mRNA quantification, integrative plasmid into NPR2 geneDepositorInsertdCAS9-VP64, mCherry, KanMX
UseInsert storage (replicative in e. coli)TagsExpressionMutationN28D in mCherry- please see depositor comments be…PromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only
