We narrowed to 4,663 results for: crispr c plasmids

1. pX330-U6-MYC-Guide-hSpCas9

   Plasmid

   #228574

   Purpose

   Expression of human MYC gRNA; CRISPR mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   MYC gRNA (MYC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

   Plasmid

   #175570

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   XbaI site added upstream of U6 promotor. G to C …

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDLX1-donor

   Plasmid

   #112343

   Purpose

   CRISPR donor plasmid to tag human transcription factor DLX1 with GFP

   Depositor

   Kevin White

   Insert

   DLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:2:172087403:G>C,hg38:2:172087433:G>A,h…

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. peft-3::cas-9::tbb-2 3'UTR

   Plasmid

   #48960

   Purpose

   this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDS246

   Plasmid

   #43861

   Purpose

   Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

   Depositor

   Keith Joung

   Insert

   mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
   

   Use

   CRISPR

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO1811_Cas9_noNLS_human

   Plasmid

   #74490

   Purpose

   Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK02

   Plasmid

   #105133

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM126

   Plasmid

   #105134

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPN10

   Plasmid

   #114386

   Purpose

   Empty gRNA

   Depositor

   Vincent Dion

   Article

   Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJW1328

    Plasmid

    #69488

    Purpose

    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Insert

    sgRNA(F+E) targeting pha-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 U6 promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTFE3-donor

    Plasmid

    #112377

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

    Depositor

    Kevin White

    Insert

    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMLX-donor

    Plasmid

    #112370

    Purpose

    CRISPR donor plasmid to tag human transcription factor MLX with GFP

    Depositor

    Kevin White

    Insert

    MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:17:42570798:G>A, hg38:17:42570950:T>G,…

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTHRB-donor

    Plasmid

    #112333

    Purpose

    CRISPR donor plasmid to tag human transcription factor THRB with GFP

    Depositor

    Kevin White

    Insert

    THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. HCP8

    Plasmid

    #166110

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5

    Depositor

    Matthias Heinemann

    Insert

    Whi5-sg106 (WHI5 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SapTrap Kit

    Plasmid Kit

    #1000000077

    Purpose

    SapTrap: modular toolkit for building CRISPR/Cas9 targeting vectors to insert genetic tags in C. elegans genome. 21 vector assembly plasmids, Unc-32::GFP targeting vector, 5 Cre/Flp expression vectors

    Depositor

    Erik Jorgensen

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

20. HCP9

    Plasmid

    #166111

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

    Depositor

    Matthias Heinemann

    Insert

    Hta2-sg18 (HTA2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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