We narrowed to 54,831 results for: ran

1. T7-CMVtrans-FFLuc-polyA

   Plasmid

   #101156

   Purpose

   Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

   Depositor

   Marcel Bruchez

   Article

   Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Banshee shRandom mCherry

   Plasmid

   #80145

   Purpose

   retroviral expression of random shRNA

   Depositor

   Ellen Rothenberg

   Insert

   random shRNA
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQC membrane TdTomato IX

   Plasmid

   #37351

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   membrane TdTomato
   

   Use

   Tags

   Palmitoylation signal*

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GFP-Grin1 KI

   Plasmid

   #131485

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin1, Grin1 Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORANGE GFP-Camk2a KI

   Plasmid

   #131484

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pORANGE GFP-Grin2a KI

   Plasmid

   #131486

   Purpose

   Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pORANGE GFP-Grin2b KI

   Plasmid

   #131487

   Purpose

   Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2b Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pORANGE Tubb3-GFP KI

   Plasmid

   #131497

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pORANGE GFP-Grin1 KI #3

   Plasmid

   #139659

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pORANGE GFP-Nlgn3 KI

    Plasmid

    #131501

    Purpose

    Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE GFP-Grin1 KI #2

    Plasmid

    #139658

    Purpose

    Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-P-RAN-GFP1

    Plasmid

    #106408

    Purpose

    P-RAN-GFP1 (RANbody GFP1::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-GFP1
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ornithine transcarbamylase (OTC)

    Plasmid

    #71877

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC (Otc Rat)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORANGE GFP-Syt7 KI

    Plasmid

    #131488

    Purpose

    Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Syt7, Syt7 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pORANGE Cplx1-GFP KI

    Plasmid

    #131475

    Purpose

    Endogenous tagging of Complexin1: C-terminal (amino acid position: L128)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cplx1, Cplx1 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pORANGE GFP-CADPS KI

    Plasmid

    #131482

    Purpose

    Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cadps Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLD1 translation factors

    Plasmid

    #117760

    Purpose

    BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 1-13
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLD3 translation factors

    Plasmid

    #117762

    Purpose

    BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

    Depositor

    Anthony Forster

    Article

    Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Translation factor cistrons 22-30
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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