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pDONR223-PAK2

Plasmid

#23655

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK2 (PAK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2

Use

Gateway Cloning

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_50

Plasmid

#109248

Purpose

incW BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pICH47772

Plasmid

#48004

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-Flag-BRD4-7D

Plasmid

#90007

Purpose

expresses 7D mutant BRD4

Depositor

Kornelia Polyak

Article

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484D, 488S to 488D, 492S to 492D,…

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1

Plasmid

#73562

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1 (ORAI1 Human, Synthetic)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-H3.3WT-FLAG/HA

Plasmid

#128740

Purpose

Expression of histone H3.3WT-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3 WT (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Promoter

MSCV

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKK-mClover3-TEV

Plasmid

#105778

Purpose

Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mClover3-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-TRIB3

Plasmid

#23677

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRIB3 (TRIB3 Human)

Use

Gateway Cloning

Mutation

P5T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
ER_K20

Plasmid

#133861

Purpose

Expression of ER-targeted Ribosome stalling reporter

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

ER_K20

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK9

Plasmid

#23459

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK9 (NEK9 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-DYRK3

Plasmid

#23635

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK3 (DYRK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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minCMV-1xmNG-dMCP

Plasmid

#231091

Purpose

Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

Depositor

John Ngo

Article

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Insert

1xmNG-dMCP

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ch-TOGKDP-GFP

Plasmid

#69112

Purpose

For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Insert

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Available Since

Oct. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

Plasmid

#67275

Purpose

For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

Depositor

Jannik Elverløv-Jakobsen

Article

Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

Insert

puromycin resistant gene

Tags

linked to red flouresent protein gene (katushka)

Expression

Mammalian

Promoter

CAG

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-PAK3

Plasmid

#23439

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK3 (PAK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAd-Track SIRT1 H355A

Plasmid

#8441

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pCEP4-PRL-3

Plasmid

#16618

Depositor

Bert Vogelstein

Article

Saha et al Science. 2001 Nov 9. 294(5545):1343-6.

Insert

PRL-3 (PTP4A3 Human)

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pL-CRISPR.SFFV.PAC

Plasmid

#57829

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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