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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 57,485 results for: tra

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Showing: 2581 - 2600 of 57485 results

  10. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  12. pEGFP-ATF6-(S1P-)

    Plasmid
    #32956
    Purpose
    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.
    Depositor
    Ron Prywes
    Article
    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-
    Insert
    ATF6 (ATF6 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S1Protease site mutation: R415A/R416A
    Promoter
    CMV
    Available Since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32956-plasmid-map-sequence-id-226826

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  16. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available Since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    62887-plasmid-map-sequence-id-105910

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  17. pSBbi-GN

    Plasmid
    #60517
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60517-plasmid-map-sequence-id-379976

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Showing: 2581 - 2600 of 57485 results