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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

Plasmid

#184397

Purpose

Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Firefly luciferase beta-globin-PTC39

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Mutation

PTC in beta-globin at amino acid position 39

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-NBRE3

Plasmid

#208254

Purpose

pGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cells

Depositor

Douglas Kojetin

Article

Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

Insert

3X NBRE-LUC

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TLR targeting vector

Plasmid

#64215

Purpose

Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

traffic light reporter (TLR) (AAVS1 Human)

Use

Human targeting

Mutation

see publication for details

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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EF1a-TET1-dCas9

Plasmid

#235593

Purpose

Tet1CD fused to N-terminus of dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)

Use

CRISPR

Tags

3xNLS, TET1 CD, and tagBFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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HIP-flash (HOP-flash mutant)

Plasmid

#83466

Purpose

Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-E SCT-T2A-GFP

Plasmid

#205461

Purpose

lentiviral plasmid for expression of HLA-E SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-E SCT (HLA-E Human)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Long P2A

Plasmid

#164109

Purpose

Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM-Mt-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-LAMP1-mCherry

Plasmid

#214274

Purpose

Mammalian expression of LAMP1-mCherry (lysosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

LAMP1-mCherry (Lamp1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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NL4-3 mCherry Luciferase

Plasmid

#44965

Purpose

HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

Depositor

Warner Greene

Article

Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371

Insert

mCherry, Luciferase, IRES

Use

Lentiviral

Tags

Luciferase, IRES and mCherry with D212G

Mutation

S34C mutation in Nef

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RYK-V5/HIS

Plasmid

#205610

Purpose

Expression of human RYK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

RYK receptor tyrosine kinase (RYK Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215326

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

dNTP63 Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

dNTP63

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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Oct4-IRES-eGFP-PGK-Neo

Plasmid

#48681

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Oct4 (Pou5f1 Mouse)

Use

CRISPR

Tags

GFP

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRKC-V5/HIS

Plasmid

#205615

Purpose

Expression of human TRKC receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TRKC receptor tyrosine kinase (NTRK3 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-GalT-mCherry

Plasmid

#214271

Purpose

Mammalian expression of GalT-mCherry (Golgi marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

GalT(1-82)-mCherry (B4GALT1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PCDNA3-GFP1-9 T2A mCherry

Plasmid

#124430

Purpose

Expresses GFP1-9 and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9 T2A mCherry

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-G SCT-T2A-GFP

Plasmid

#205462

Purpose

lentiviral plasmid for expression of HLA-G SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-G SCT (HLA-G Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AMPK(T>A)-SPARK

Plasmid

#226256

Purpose

phospho-null mutant of AMPK-SPARK

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

AMPK(T>A)-SPARK

Expression

Mammalian

Mutation

substituted Threonine 20 with Alanine

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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