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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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167,433 results

Showing: 2621 - 2640 of 167433 results

  1. SSTR4-DuET

    Plasmid
    #213379
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR4 (SSTR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213379-plasmid-map-sequence-id-430891

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  2. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid
    #207158
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAS (GNAS Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(260)N
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207158-plasmid-map-sequence-id-410280

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  8. pD649-HAsp-CEACAM6-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156723
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CEACAM6 (CEACAM6 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156723-plasmid-map-sequence-id-302555

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  9. pD649-HAsp-CEACAM5-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156937
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CEACAM5 (CEACAM5 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156937-plasmid-map-sequence-id-302983

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  10. pD649-HAsp-CEACAM8-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156730
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CEACAM8 (CEACAM8 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156730-plasmid-map-sequence-id-382016

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  12. pcDNA3.1(-) HuTF-Fc-His tag

    Plasmid
    #183253
    Purpose
    Soluble expression of the extracellular domains of human tissue factor (TF)-Fc
    Depositor
    Alexander McLellan
    Insert
    Tissue factor (F3 Human)
    Tags
    8xHis
    Expression
    Mammalian
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183253-plasmid-map-sequence-id-369717

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  15. MEK1-DD-V5

    Plasmid
    #217298
    Purpose
    Lentivirus vector for expression of MEK1 S218D/S222D
    Depositor
    Channing Der
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    V5
    Mutation
    S218D/S222D
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    217298-plasmid-map-sequence-id-441105

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  17. pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)

    Viral Prep
    #100798-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Article
    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 2621 - 2640 of 167433 results