We narrowed to 11,219 results for: NSI

1. lentiCRISPRv2-TTF-1-gRNA

   Plasmid

   #101857

   Purpose

   Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

   Depositor

   David Mu

   Article

   Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

   Insert

   gRNA targeting TTF-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA FLAG TORC3

   Plasmid

   #22976

   Depositor

   Marc Montminy

   Article

   Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

   Insert

   TORC3 (CRTC3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pBAD-HisD-KRaION2

   Plasmid

   #177800

   Purpose

   Bacterial expression of green fluorescent potassium indicator KRaION2

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   KRaION2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187922

   Purpose

   Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase acidic protein
   

   Use

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJA167

   Plasmid

   #128536

   Purpose

   Expresses DsRed

   Depositor

   Marcos Alcocer

   Article

   Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

   Insert

   NFAT-DsRed reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40-NFAT

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

   Plasmid

   #191159

   Purpose

   Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mTagBFP:EGFP.s2:mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB092a

   Plasmid

   #79217

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1+2); Ppro1

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRKVSV Rac

   Plasmid

   #50535

   Depositor

   Kenneth Yamada

   Article

   Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

   Insert

   small GTPase (RAC1 Human)
   

   Use

   Tags

   2X VSV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLicC-MBP-APETx2

   Plasmid

   #72668

   Purpose

   Periplasmic expression of APETx2 in E.coli

   Depositor

   Glenn King

   Article

   Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

   Insert

   APETx2
   

   Use

   Tags

   His tag, MBP, MalE signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL

    Plasmid

    #161783

    Purpose

    Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and hPGK

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMVie-IRES-BlastR alt MCS

    Plasmid

    #120862

    Purpose

    3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

    Depositor

    Ghassan Mouneimne

    Article

    Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET_SZ1-RGG-RGG

    Plasmid

    #124942

    Purpose

    Expression of SZ1-RGG-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Fyn-Fyn Biosensor

    Plasmid

    #124655

    Purpose

    FRET biosensor. Fyn-tagged biosensor targeting to membrane

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYFP-NGAT

    Plasmid

    #11383

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204794

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Use

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-CreiLOV-P2A-FusionRed

    Plasmid

    #192818

    Purpose

    Expresses CreiLOV with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYFAC-CH3

    Plasmid

    #168979

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRX-INU-TEX264 LIR4A-FLAG

    Plasmid

    #128259

    Purpose

    Expresses TEX264 LIR4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 LIR4A (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed FEEL (aa 273-276) to alanines

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAB107a

    Plasmid

    #79218

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR-SNAP-PD-1

    Plasmid

    #223572

    Purpose

    Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS1422

    Plasmid

    #12548

    Depositor

    Robert Sobol

    Article

    Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

    Insert

    human Methylpurine DNA glycosylase (MPG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a_Pro-IL-36γ

    Plasmid

    #214345

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36G (IL36G Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDest-521

    Plasmid

    #159688

    Purpose

    Gateway destination vector for E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLuciferase (GB0096)

    Plasmid

    #68201

    Purpose

    Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luciferase coding region
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAV-U6+27-Tornado-F30-Squash-Broccoli

    Plasmid

    #177918

    Purpose

    Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Squash-Broccoli
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUDE1084

    Plasmid

    #168500

    Purpose

    expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

    Depositor

    Jean-Marc Daran

    Article

    Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

    Inserts

    Fncas12a
    T7RNA polymerase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TDH3 and TEF1

    Available since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-nef-CIAO2-TVA-mCherry

    Plasmid

    #149297

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Start codon removed.

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAB078d9

    Plasmid

    #79207

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1+2+3); Ppro1

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAB088h

    Plasmid

    #79215

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PXPR007 sgGFP-1

    Plasmid

    #74960

    Purpose

    Cas9 + sgGFP-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHDAdVenus-geo-TK

    Plasmid

    #26423

    Depositor

    Kohnosuke Mitani

    Article

    Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    PGK-b-geo, HSV-TK, CAG-Venus
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHygromycin (GB0211)

    Plasmid

    #68204

    Purpose

    Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hygromycin resistance gene coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCB169

    Plasmid

    #37390

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    dynein light chain TcTex-type 3 (DYNLT3 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMTMVi-N

    Plasmid

    #118756

    Purpose

    Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.

    Depositor

    Jose-Antonio Daros

    Article

    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

    Insert

    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    

    Use

    Intermediate to build tobacco mosaic virus recomb…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAB085d

    Plasmid

    #79209

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ptet

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-ExRai-CKAR(T/A)

    Plasmid

    #118410

    Purpose

    Negative-control mutant for ExRai-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140222

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JW617

    Plasmid

    #64809

    Purpose

    CUC3 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain and Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge