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pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rac1 (Rac1 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRC05

Plasmid

#114684

Purpose

FKBP-TEV-C

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FKBP-TEV-C

Tags

GST

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBM13h-sd4U

Plasmid

#179690

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N wildtype

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-STIL

Plasmid

#80265

Purpose

baculovirus creation plasmid for GST-STIL

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pRC04

Plasmid

#114683

Purpose

FRB-TEV-N

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FRB-TEV-N

Tags

MBP

Expression

Bacterial

Promoter

Tac Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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peSpdCas9-J23109

Plasmid

#113153

Purpose

Plasmid constitutively expressing eSpdCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpdCas9

Expression

Bacterial

Mutation

D10A & K810A & H840A & K1003A & R…

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-Cre.ape

Plasmid

#71749

Purpose

U+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Cdc12-Cdc11

Plasmid

#131162

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

6his-Cdc12 + Cdc11

Use

Bicistronic

Tags

Cdc11: S-Tag and Cdc12: 6his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10

Plasmid

#131160

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10

Use

Bicistronic

Tags

Cdc10: S Tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA4

Plasmid

#174034

Purpose

In vitro transcription of anti-CRISPR AcrIIA4 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA4

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-AcrIIA2

Plasmid

#174033

Purpose

In vitro transcription of anti-CRISPR AcrIIA2 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

AcrIIA2

Use

In vitro transcription

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJL006

Plasmid

#92329

Purpose

Express mEos3.2 tagged FtsZD212A mutant in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Mutation

D212A

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-DH

Plasmid

#67898

Purpose

Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-DH

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2

Plasmid

#111698

Purpose

Theophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaST1

Plasmid

#22632

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST1 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST1 domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pC.059

Plasmid

#119564

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Ptrc10

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-I-K-CAAX

Plasmid

#41643

Purpose

Catalytically active control, intersectin Dbl homology (DH) domain fused to wild-type Dronpa

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

IntersectinDH-Dronpa145K-CAAX

Tags

CAAX box

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

Plasmid

#132638

Purpose

expresses mouse Tankyrase1 5ARC (171–961) in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

Insert

Tankyrase1 (171–961)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Lmo4.2 shRNA

Plasmid

#59293

Purpose

shRNA to mouse Lmo4

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

Insert

Lmo4.2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pLL3.7 Lmo4.3 shRNA

Plasmid

#59292

Purpose

shRNA to mouse Lmo4

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

Insert

Lmo4.3 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
MDH1-miR-181a1-H2Kb2

Plasmid

#17847

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

H-2Kb (H2-K1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pBN79

Plasmid

#86717

Purpose

DamID plasmid for C. elegans

Depositor

Peter Askjaer

Article

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::Dam::MYC::Emr-1

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPRP3 C3(1) promoter

Plasmid

#12490

Depositor

Jeffrey Green

Article

Shibata et al Cancer Gene Ther. 2001 Jan . 8(1):23-35.

Insert

C3(1) promoter (C3 Rat)

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pRS306:Met3p-mCherry-Tub1

Plasmid

#37554

Depositor

Wei-Lih Lee

Article

Markus et al Cytoskeleton (Hoboken). 2011 Mar;68(3):1

Insert

Met3p-mCherry-Tub1

Expression

Bacterial

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
SMAD4 KO Neo

Plasmid

#16646

Depositor

Bert Vogelstein

Article

Zawel et al Proc Natl Acad Sci U S A. 2002 Mar 5. 99

Insert

Smad4 knock-out construct (SMAD4 Human)

Expression

Bacterial

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-BirA

Plasmid

#71752

Purpose

Aurein1.2+36GFP-BirA fusion for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2+36GFP-BirA

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGAD424-rSyntenin1

Plasmid

#89425

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Tags

Gal4 AD

Expression

Yeast

Promoter

ADH1

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S502A

Plasmid

#19281

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCS2 xRor2-EGFP

Plasmid

#67163

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

EGFP

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pGEXndoE(E662Q)

Plasmid

#47716

Purpose

Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

Depositor

Mattias Collin, Vincent Fischetti

Article

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Tags

GST-EndoE(E186Q) and Glutathione S-transferase

Expression

Bacterial

Mutation

Changed Glu-662 to Gln

Promoter

tac

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a-mRor2deltaST2

Plasmid

#22633

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST2 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST2 domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-rsAKARev

Plasmid

#166231

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker (rsAKARev) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P5SHb + STS:P6SHb

Plasmid

#139792

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P5SHb
STS:P6SHb

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S + STS:AP4S

Plasmid

#139795

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL:P3S
STS:AP4S

Expression

Bacterial

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NmCherry-SpVasa

Plasmid

#66786

Purpose

expression of mCherry in Sea Urchin germ cells

Depositor

Amro Hamdoun

Article

Campanale et al Development. 2012 Feb;139(4):783-92. doi

Insert

Vasa

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pB24

Plasmid

#61153

Purpose

Expression of Hnt1

Depositor

Charles Brenner

Article

Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

Insert

Hnt1 (HNT1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-XIAPmut

Plasmid

#8341

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Tags

GST

Expression

Bacterial

Mutation

H467A

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only
DPY-pNCS

Plasmid

#17365

Depositor

Stephen Goff

Article

Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

Insert

MMLV proviral DNA

Mutation

deletion that removes 5 amino acids (DPPPY) from …

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
XE86 TM Delta N Beta-catenin GFP

Plasmid

#16833

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

TM Delta N beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only

Showing: 5241 - 5280 of 26655 results

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