-
Plasmid#152063PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134048
Plasmid#152049PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046344
Plasmid#149003PurposeYeast Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAS-mCherry-NLS
Plasmid#87695PurposeGal4VP16 driven expression of mCherry-NLS in mammalian cellsDepositorInsertmCherry-NLS
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF1389
Plasmid#104962PurposeBacterial expression plasmid of bdSENP1 proteaseDepositorInsertSENP1
UseTagsHis14 tag and Tev protease cleavage siteExpressionBacterialMutationPromoterlacUV5Available sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPSup
Plasmid#149416PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRExpress
Plasmid#122486PurposeA non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)DepositorInsertsLacZ
poly-PRE
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-Destination
Plasmid#37184PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.DepositorTypeEmpty backboneUseTagsFokI nucleaseExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPSint
Plasmid#149421PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEQ1007
Plasmid#83938Purposeexpress human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cellsDepositorInsertIRS1
UseTags6xHISExpressionMammalianMutationR833L in IRS1 (please see depositor comment)PromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLM802
Plasmid#27203DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMLM800
Plasmid#27202DepositorInsertFokI endonuclease
UseZebrafish expressionTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEwt2/Imwt2
Plasmid#107127Purposeplasmid for expression of WT colicin endonuclease and immunity pair 2DepositorInsertcolEwt2/Imwt2
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN1A-c001
Plasmid#175491PurposeProtein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.DepositorInsertCAPN1 / CAPNS1 (CAPN1 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM0531
Plasmid#128988PurposePart plasmid for VP16-CIB1, Type 3 (coding sequence) partDepositorInsertSV40NLS-VP16-CIB1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0528
Plasmid#128985PurposePart plasmid for pZF(3BS), Type 2 (promoter) partDepositorInsertpZF(3BS)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPSdown
Plasmid#149417PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the 3'-UTR of the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSup2
Plasmid#149418PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
XX01CAPN2A-c001
Plasmid#175492PurposeProtein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.DepositorInsertCAPN2 / CAPNS1 (CAPN2 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSdown2
Plasmid#149419PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only