We narrowed to 15,788 results for: LEA

1. pcDNA3.1-hSyncytin-1(AAAR)

   Plasmid

   #182442

   Purpose

   hSyncytin-1, furin cleavage site RNKR>AAAR(314-317)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   furin cleacage site RNKR>>AAAR(314-317)

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7CFE1_EVD68_VP12A_C107R

   Plasmid

   #212982

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A C107R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed cysteine 107 from 2A to Arginine

   Promoter

   T7

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Cdc45-mCer

   Plasmid

   #211389

   Purpose

   mammalian expression of human Cdc45 C-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Cdc45 (CDC45 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7CFE1_EVD68_3ABC_C147R

   Plasmid

   #212978

   Purpose

   Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed Cysteine 147 in 3C to Arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7CFE1_EVD68_3ABCD_C147R

   Plasmid

   #212980

   Purpose

   Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABCD C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed cysteine 147 from 3C to arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7CFE1_EVD68_VP12A_N84T

   Plasmid

   #212983

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A N84T
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed Asparagine 84 from 2A to Threonine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-C17orf53-mCer

   Plasmid

   #211420

   Purpose

   mammalian expression of human C17orf53 C-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   C17orf53 (HROB Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TALEN_C18

   Plasmid

   #47974

   Purpose

   Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI

   Depositor

   Gang Bao

   Insert

   Transcription Activator Like Effector Nuclease
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Shortened C-Terminus,Codon Optimized FokI Endonuc…

   Promoter

   CMV

   Available since

   Dec. 12, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pPS1082

   Plasmid

   #8910

   Depositor

   Pamela Silver

   Article

   Taura et al J Biol Chem. 1997 Dec 12. 272(50):31877-

   Insert

   YRB2 (YRB2 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pPS2168

    Plasmid

    #1366

    Depositor

    Pamela Silver

    Article

    Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

    Insert

    RPL11B (RPL11B Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-hNurr1-NE

    Plasmid

    #102363

    Purpose

    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

    Depositor

    Shu Leong Ho

    Insert

    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    

    Use

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIPZ

    Plasmid

    #206981

    Purpose

    empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCytochrome C-GFP

    Plasmid

    #41182

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-CrmA

    Plasmid

    #11832

    Depositor

    Guy Salvesen

    Article

    Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6.

    Insert

    CrmA (CrmA Cowpox virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKmyc-Exp5

    Plasmid

    #12552

    Depositor

    Ian Macara

    Article

    Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

    Insert

    Exportin-5 (XPO5 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Oct. 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ec dnaN-pHK/P

    Plasmid

    #237239

    Purpose

    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)

    Depositor

    Michael O'Donnell

    Insert

    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    

    Use

    Tags

    his-10+PKA kinase motif+PreScission Protease clea…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pG1K

    Plasmid

    #71741

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pG2K

    Plasmid

    #71742

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3-K230-FokI_L (DAS)

    Plasmid

    #32546

    Depositor

    Jin-Soo Kim

    Article

    Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

    Insert

    ZFN
    

    Use

    Tags

    HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Dec. 2, 2011

    Availability

    Academic Institutions and Nonprofits only