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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,273 results for: cas9

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Showing: 2621 - 2640 of 14273 results

  5. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  6. AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

    Viral Prep
    #107312-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hui Yang
    Article
    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/
    Tags
    mCherry (physically separate, not a fusion protein)
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. HiFi dFE

    Plasmid
    #190142
    Purpose
    Expresses HiFiCas9-T4pol (HiFi dFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    HiFi Cas9-T4pol (HiFi dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190142-plasmid-map-sequence-id-374983

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  12. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  15. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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  17. pUC19-B2KB46

    Plasmid
    #103086
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B2KB46(Cas9 coding gene from Elusimicrobium minutum (strain Pei191))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    May 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103086-plasmid-map-sequence-id-209052

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  18. pUC19-K0XCK7

    Plasmid
    #103118
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    K0XCK7(Cas9 coding gene from Barnesiella intestinihominis YIT 11860)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103118-plasmid-map-sequence-id-201880

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  19. pUC19-H1DEI0

    Plasmid
    #103113
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    H1DEI0(Cas9 coding gene from Odoribacter laneus YIT 12061)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103113-plasmid-map-sequence-id-201872

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Showing: 2621 - 2640 of 14273 results