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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,564 results for: CHI

Showing: 6651 - 6700 of 23564 results

  1. pST50Trc4-FLAGHISNDHFR

    Plasmid
    #64001
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64001-plasmid-map-sequence-id-177554

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  2. pST50Trc4-FLAGDHFR

    Plasmid
    #63992
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63992-plasmid-map-sequence-id-177398

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  3. pST50Trc3-HISDHFR

    Plasmid
    #63971
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63971-plasmid-map-sequence-id-177508

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  4. pST50Trc4-DHFRSTR

    Plasmid
    #64004
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    Strep II (STR)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64004-plasmid-map-sequence-id-177410

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  5. pST50Trc4-FLAGNDHFR

    Plasmid
    #63997
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63997-plasmid-map-sequence-id-177403

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  9. pTH09

    Plasmid
    #70064
    Purpose
    artificial transcription template "terminator insertion"
    Depositor
    Jeff Gelles
    Article
    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi
    Insert
    lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pST50Trc2-FLAGDHFR

    Plasmid
    #63956
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63956-plasmid-map-sequence-id-177362

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  11. pST50Trc2-FLAGHISNDHFR

    Plasmid
    #63965
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63965-plasmid-map-sequence-id-177498

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  24. pSF18

    Plasmid
    #222593
    Purpose
    BB2 ZWF1 tet-on overexpression cassette
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222593-plasmid-map-sequence-id-443809

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  25. pNY-346

    Plasmid
    #231046
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to human Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231046-plasmid-map-sequence-id-458329

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  26. pVP293

    Plasmid
    #222062
    Purpose
    GreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Insert
    Filler
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Tags
    Expression
    Mutation
    Backbone Esp3i sites have been domesticated
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222062-plasmid-map-sequence-id-437925

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  27. pVP294

    Plasmid
    #222063
    Purpose
    GreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Insert
    Filler
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Tags
    Expression
    Mutation
    Backbone Esp3i sites have been domesticated
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222063-plasmid-map-sequence-id-447319

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  28. pVP076

    Plasmid
    #222028
    Purpose
    Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Tags
    Expression
    Mutation
    All Esp3i sites domesticated
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222028-plasmid-map-sequence-id-447309

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  29. pVP077

    Plasmid
    #222029
    Purpose
    Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Tags
    Expression
    Mutation
    All Esp3i sites domesticated
    Promoter
    Available since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222029-plasmid-map-sequence-id-447312

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  33. pIA036

    Plasmid
    #200425
    Purpose
    amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    bsdronPA
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    200425-plasmid-map-sequence-id-443100

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  37. pGU08

    Plasmid
    #212149
    Purpose
    Plasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.
    Depositor
    Kang Zhou
    Insert
    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212149-plasmid-map-sequence-id-424775

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  40. INT_Cargo_PgyrA_katG_dadA6

    Plasmid
    #212142
    Purpose
    Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212142-plasmid-map-sequence-id-420769

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  42. pHIT198

    Plasmid
    #204509
    Purpose
    pET-derived vector for expression of proteins N-terminally tagged with 6xHis and maltose binding protein (multi-cloning sites: NdeI, NotI, SpeI, SalI, PstI, SgfI, and XhoI)
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Type
    Empty backbone
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204509-plasmid-map-sequence-id-403818

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Showing: 6651 - 6700 of 23564 results