We narrowed to 32,166 results for: uros

1. pBabe puro pI3K p110 CAAX

   Plasmid

   #13339

   Depositor

   Channing Der

   Article

   McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

   Insert

   pI3K p110 CAAX (PIK3CA Bovine)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 20, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pLVX-HA-UBE3B(C1036A)-IRES-Puro

   Plasmid

   #107348

   Purpose

   Expresses HA tagged UBE3B mutant C1036A in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B(C1036A) (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Cysteine 1036 to Alanine

   Promoter

   CMV

   Available Since

   April 19, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pUC57-PB-PGK-tetR-KRAB-2A-Puro

   Plasmid

   #129536

   Purpose

   PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   PB-PGK-tetR-KRAB-2A-Puro
   

   Use

   Unspecified

   Promoter

   hPGK promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro H-Ras G12V Y40C

   Plasmid

   #12276

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V Y40C (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The Y40C mutat…

   Available Since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. Mis12-targted Aurora B FRET sensor

   Plasmid

   #45231

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   Mis12 targeted Aurora B FRET sensor
   

   Expression

   Mammalian

   Available Since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-puro U6 sgRNA SOX17 -177

   Plasmid

   #50925

   Purpose

   U6 driven sgRNA targeting Sox17 -177 bp from TSS

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Sox17 -177 sgRNA (SOX17 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3puro-mGFP-ROCK2-C (941-1388)

   Plasmid

   #225471

   Purpose

   Express mEGFP-fusion protein of ROCK2 fragment (941-1388)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   ROCK2 (ROCK2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   C terminal fragment (941-1388)

   Promoter

   CMV

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Flag-Polß(WT)-IRES-puro

   Plasmid

   #128653

   Purpose

   mammalian expression of Flag-Polß(WT) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

   Plasmid

   #162098

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to HA-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   HA-FLAG-AU1

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe puro-HA-PIK3CA(H1047R;I848V)

    Plasmid

    #33253

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and isoleucine…

    Promoter

    SV40

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pBABE-puro-Halo-hMRE11(H129N)

    Plasmid

    #208394

    Purpose

    To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Mutation

    H129N

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLC-CUL4A K705R-P2A-Puro

    Plasmid

    #124305

    Purpose

    Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Lysine 705 to Arginine

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid

    #68345

    Purpose

    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

    Inserts

    puromycin
    hCas9
    

    Use

    CRISPR; Flp/frt

    Tags

    linked to red flouresent protein gene mKateII and…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-puro/HA-FLAG-RBP2 H483A

    Plasmid

    #14803

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    changed His 483 to Ala

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-TRE3G-BE3-PGK-Puro

    Plasmid

    #110845

    Purpose

    Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    TRE3G

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetroX-TetOne-Puro-p62 deltaUBA

    Plasmid

    #204550

    Purpose

    Expresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    deltaUBA

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. shPBRM1 #122-Tet-pLKO-puro

    Plasmid

    #107401

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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