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Showing: 6701 - 6750 of 11732 results

  6. pCAGEN-ActinVhh-sfGFP-P2A-HA-tdiRFP-caax (JDW 1532)

    Plasmid
    #242606
    Purpose
    A CAGGS driven expression vector containing an actin nanobody fused to sfGFP to label actin followed by a HA tagged tdiRFP fused to a caax tag to label the cell membrane.
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    Actin Vhh-sfGFP-P2A-HA-tdiRFP-caax
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242606-plasmid-map-sequence-id-495400

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  12. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid
    #216309
    Purpose
    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216309-plasmid-map-sequence-id-433372

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  16. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid
    #207636
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207636-plasmid-map-sequence-id-409876

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  21. pcDNA3.1_mGFP-FGFR3-K650Q

    Plasmid
    #191756
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1948A>C (p.K650Q) + silent mutation c.1938C&…
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191756-plasmid-map-sequence-id-382285

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  36. FB003

    Plasmid
    #119677
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119677-plasmid-map-sequence-id-238439

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  42. PARP14 (PARP14A-c013)

    Plasmid
    #110254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP14 (PARP14 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110254-plasmid-map-sequence-id-212336

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  43. pUC19-E3HCA8

    Plasmid
    #103100
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103100-plasmid-map-sequence-id-209053

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  44. pUC19-D6GRK4

    Plasmid
    #103097
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    D6GRK4(Cas9 coding gene from Filifactor alocis (strain ATCC 35896 / D40 B5))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103097-plasmid-map-sequence-id-201807

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  45. pAGH42

    Plasmid
    #107249
    Purpose
    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    vipp1-gfp
    Use
    Suicide vector for destined for double homologous…
    Tags
    mGFPmut3
    Expression
    Bacterial
    Promoter
    native promoter of sll0617
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107249-plasmid-map-sequence-id-207556

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Showing: 6701 - 6750 of 11732 results