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pcDNA5-Flag/HA-TRBP-L326A/Y358A

Plasmid

#215892

Purpose

Expression of TRBP-L326A/Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A/Y358A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS gag-gfp/vpx

Plasmid

#115808

Purpose

SFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172505

Purpose

Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBLO 64.1

Plasmid

#123120

Purpose

E. coli expression vector for active deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Promoter

NA

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1709A)

Plasmid

#41588

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1709A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pscALPS-HIV-1 Group O-vpr

Plasmid

#115836

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group O Vpr (L20571)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-E

Plasmid

#215814

Purpose

Expresses SARS-CoV-2 Envelope protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Envelope (E )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-M

Plasmid

#215813

Purpose

Expresses SARS-CoV-2 Membrane protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Membrane (M )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMT2-HA-MKK3

Plasmid

#21580

Depositor

John Kyriakis

Article

Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-

Insert

mitogen-activated protein kinase kinase 3 (MAP2K3 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-SUV39h1 ts2

Plasmid

#115881

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-loxp hM4Di-mCherry

Plasmid

#161581

Purpose

Expression of hM4Di-mCherry in a cre-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Mutation

NA

Promoter

hSyn

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts1

Plasmid

#115880

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-CPZ-TAN3 vpr

Plasmid

#115833

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-TAN3 Vpr (DQ374658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (K70A)

Plasmid

#41589

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

K70A

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
pscALPS-SIV-CPZ-LB7 vpr

Plasmid

#115834

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-LB7 Vpr (DQ373064)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GOR -vpr

Plasmid

#115835

Purpose

Encodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGOR Vpr (FJ424871)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts1

Plasmid

#115886

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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