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CHIKV eGFP-Zeo replicon

Plasmid

#249289

Purpose

SP6-driven CHIKV_LR2006 OPY1 replicon with eGFP-Zeocin reporter selection cassette

Depositor

Amy Kistler

Insert

CHIKV LR2006-OPY1 replicon harboring nonstructural genes (nsp1-nsp4) and eGFP-Zeocin ORF in place of structural genes

Expression

Bacterial

Mutation

structural polyprotein open reading frame deleted…

Promoter

SP6

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pUbC-nls-ha-stdMCP-stdGFP

Plasmid

#98916

Purpose

synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

Depositor

Robert Singer

Article

Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

Insert

stdMCP-stdGFP

Use

Lentiviral

Tags

GFP

Promoter

UbC

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1-alpha-V

Plasmid

#27290

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha V (ITGAV Human)

Expression

Mammalian

Mutation

Isoform 2

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only

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Human PNP his-tagged

Plasmid

#64076

Purpose

Expression of human Purine Nucleoside Phosphorylase

Depositor

Vern Schramm

Article

Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
nontarget S. pyogenes scaffold CARGO array

Plasmid

#191319

Purpose

Contains an array of nontargeting guide RNAs

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. pyogenes scaffold CARGO array

Plasmid

#191316

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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p8092 LentiCRISPR v2 sgNT-1

Plasmid

#163315

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgNT-1

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDRF1 mScarlet3-T2A-mTurquoise2

Plasmid

#219848

Purpose

equimolar expression of mScarlet3 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

mScarlet3-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDY1935

Plasmid

#212315

Purpose

Express ApmFnuc protein with sumotag

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Isvmimi

Tags

6xHis-thrombin site-TwinStrepTag-SUMO

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-mito

Plasmid

#113919

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-cyto

Plasmid

#113917

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-SpCas9mSA-His

Plasmid

#182951

Purpose

Express SpCas9mSA in bacteria

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

NLS-SpCas9mSA-His

Use

CRISPR

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

Plasmid

#74317

Purpose

A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

Depositor

Martin Meyer, Stephen Smith

Article

Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

Inserts

synaptophysin
DsRedExpress

Tags

EGFP

Promoter

5UAS

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymBaojin-T2A-mCherry

Plasmid

#219857

Purpose

equimolar expression of ymBaojin and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

ymBaojin-T2A-mCherry

Expression

Yeast

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eNOS-GFP

Plasmid

#22444

Depositor

William Sessa

Article

Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

Insert

WT eNOS-GFP

Tags

GFP

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pET30a-GlmU-NahK-ET64

Plasmid

#234663

Purpose

Expression an ELP-tagged fusion enzyme of GlmU and NahK

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

N-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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