We narrowed to 23,628 results for: crispr

1. pKozak_mTagBFP2-T2A-sGFP1-10_synCoTC

   Plasmid

   #199494

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

   Plasmid

   #199498

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSA_0_mTagBFP2-T2A-sGFP1-10_synCoTC

   Plasmid

   #199475

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSA_1_mTagBFP2-T2A-sGFP1-10_synCoTC

   Plasmid

   #199476

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSA_2_mTagBFP2-T2A-sGFP1-10_synCoTC

   Plasmid

   #199477

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   mTagBFP2-T2A-sGFP1-10
   

   Use

   CRISPR

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

   Plasmid

   #159906

   Purpose

   Mutagenesis of Dcc

   Depositor

   Larry Zweifel

   Insert

   Dcc (Dcc Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

   Plasmid

   #132927

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Insert

   humanized SpCas9
   

   Use

   CRISPR and Synthetic Biology; Pmagic gateway entr…

   Available Since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNR2F1.1.0-gDNA

   Plasmid

   #112484

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1

   Depositor

   Kevin White

   Insert

   NR2F1 (NR2F1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pELF4.1.0-gDNA

   Plasmid

   #112467

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELF4

   Depositor

   Kevin White

   Insert

   ELF4 (ELF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRPR1_g2gRNA_RPR1t

    Plasmid

    #64388

    Purpose

    encodes g2 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    g2 gRNA
    

    Expression

    Yeast

    Promoter

    pRPR1

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pXPR_011

    Plasmid

    #59702

    Purpose

    This lentiviral vector can be used to assay Cas9 activity.

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

    Insert

    EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ152

    Plasmid

    #69303

    Purpose

    Gateway entry vector with pco-dCas9-VP64

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-VP64 (Plant codon-optimized)
    

    Use

    CRISPR

    Mutation

    VP64 activator fusion; NLS fusion

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAS9cd

    Plasmid

    #141252

    Purpose

    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFYF1320 EGFP Site#1

    Plasmid

    #47511

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJYS2cm_crtYf

    Plasmid

    #85543

    Purpose

    Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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