We narrowed to 29,332 results for: form

1. p-preSufI(IAC)

   Plasmid

   #168516

   Purpose

   Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   pre-SufI(IAC)
   

   Tags

   6xHisC tag and HSV tag

   Expression

   Bacterial

   Mutation

   C17I and C295A

   Promoter

   T7

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NK85

   Plasmid

   #176611

   Purpose

   To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.

   Depositor

   Meghan Morrissey

   Article

   Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

   Insert

   FCERG
   

   Use

   Lentiviral

   Tags

   EGFP and HA, SNAP-tag

   Expression

   Mammalian

   Mutation

   C25A

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. C413-E40: attB4-EFSp>-attB1r

   Plasmid

   #162928

   Purpose

   Gateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   short form of human EF1 alpha promoter
   

   Use

   Synthetic Biology

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK274

   Plasmid

   #71564

   Purpose

   Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast

   Depositor

   T. J. Kappock

   Article

   Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

   Insert

   delta12 fatty acid desaturase (form 3)
   

   Expression

   Bacterial and Yeast

   Promoter

   GAL1 promoter

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSP64T3 BMP2 pro dnmVg1 (DM#182)

   Plasmid

   #15074

   Depositor

   Douglas Melton

   Article

   Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

   Insert

   BMP2-Vg1 dom neg
   

   Use

   Sp6 expression for use in xenopus

   Mutation

   dominant negative version of BMP2-Vg1 chimera. Th…

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

6. Anti-APP [6E10-A5] (Recombinant Antibody)

   Antibody

   #226376-rAb

   Purpose

   Anti-APP (Human) recombinant mouse monoclonal antibody

   Depositor

   Melina Fan

   Article

   Goepfert et al Immunity. 2020 Mar 17;52(3):499-512.e5.

   Recommended Applications

   Immunohistochemistry

   Reactivity

   Human and Mouse

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available Since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

7. XA106+pRCPam

   Bacterial Strain

   #195858

   Purpose

   E. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

8. XA103+pRCPam

   Bacterial Strain

   #195857

   Purpose

   E. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

9. XA101+pRCPam

   Bacterial Strain

   #195856

   Purpose

   E. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Bacterial Resistance

   Chloramphenicol

   Species

   Acropora millepora

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

10. XA92+pRCPam

    Bacterial Strain

    #195855

    Purpose

    E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Bacterial Resistance

    Chloramphenicol

    Species

    Acropora millepora

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-ERT2CreERT2

    Plasmid

    #13777

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    ERT2-Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-ISceID44A

    Plasmid

    #59424

    Purpose

    Expresses a catalytically inactive form of the endonuclease ISceI

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    endonuclease ISceI mutant (I-SceI )
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed aspartate 44 to alanine (D44A)

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mScarlet-Rab9aS21N

    Plasmid

    #169073

    Purpose

    Express human dominant negative form of Rab9a(S21N) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab9a (RAB9A Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSANG10-3F

    Plasmid

    #39264

    Depositor

    John McCafferty

    Article

    Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

    Type

    Empty backbone

    Use

    Bacterial expression, bacterial expression of re…

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDAI-SceI

    Plasmid

    #32061

    Purpose

    Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

    Depositor

    Miguel Valvano

    Article

    Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

    Insert

    I-SceI nuclease
    

    Expression

    Bacterial

    Promoter

    Pdhfr

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET24c(+)-mtEFTu

    Plasmid

    #30535

    Depositor

    Linda Spremulli

    Article

    Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

    Insert

    Mature bovine mitochondrial elongation factor Tu
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mature form amino acids 44-452. Mito import seque…

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSG5 E107K T Ag

    Plasmid

    #133710

    Purpose

    Large T antigen mutant form in pSG5 vector for mammalian expression.

    Depositor

    James A. DeCaprio

    Article

    Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

    Insert

    SV40gp6 (SV40gp6 SV40)
    

    Expression

    Mammalian

    Mutation

    E107K

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK370

    Plasmid

    #73595

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 2)
    

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Silent mutation in Arg299 codon.

    Promoter

    GAL1 and GAL10

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-TPNOX

    Plasmid

    #87853

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    TPNOX
    

    Tags

    C-terminal FLAG tag with a linker

    Expression

    Bacterial

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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