Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,788 results for: golden gate

Clear keywords
Showing: 2721 - 2740 of 5788 results

  1. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Publication
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

    Enlarge

  3. pECNUS2

    Plasmid
    #184885
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Publication
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    184885-plasmid-map-sequence-id-435252

    Enlarge

  4. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

    Enlarge

  5. pECNUS3

    Plasmid
    #184886
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Publication
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184886-plasmid-map-sequence-id-437894

    Enlarge

  6. pECNUS1

    Plasmid
    #184884
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Publication
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184884-plasmid-map-sequence-id-437893

    Enlarge

  8. UbiGate Components (set1)

    Plasmid kit
    #1000000144
    Purpose
    UbiGate Components (set 1) enables the reconstitution of the ubiquitination cascades of different organisms, including A. thaliana, in E. coli by 'Golden Gate' cloning.
    Depositor
    Marco Trujillo
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  11. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid kit
    #1000000138
    Purpose
    This toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression.
    Depositor
    Elena Caro Bernat
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (GoldenBraid)
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. Mobius Assembly Vector Toolkit

    Plasmid kit
    #1000000134
    Purpose
    Mobius Assembly is a Golden Gate-based cloning framework which renders both high cloning capacity and vector toolkit simplicity.
    Depositor
    Naomi Nakayama
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. The Xenorhabdus griffiniae part library

    Plasmid kit
    #1000000274
    Purpose
    Use this parts library for Golden Gate and Gibson assemblies to express DNA parts in the bacterium Xenorhabdus griffiniae.
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    Feb. 13, 2026
    Availability
    Academic Institutions and Nonprofits only

  14. FusX TALEN assembly system

    Plasmid kit
    #1000000063
    Purpose
    Streamlined Golden Gate TALE assembly of 15–16 RVD TALEN inserts in one step that is backwards compatible with popular TALE backbones for expression in multiple systems; requires additional plasmids for use.
    Depositor
    Stephen Ekker
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Zebrafish Expression
    Editing type
    TALEN
    Cloning type
    Golden Gate
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. Ekker Lab TALEN kit

    Plasmid kit
    #1000000038
    Purpose
    Complete collection for assembly of highly active 15-RVD GoldyTALENs for expression in zebrafish. The collection complements the Golden Gate TALEN and TAL Effector Kit 2.0 (#1000000024).
    Depositor
    Stephen Ekker
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Zebrafish Expression
    Editing type
    TALEN
    Cloning type
    Golden Gate
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. UbiGate E2 activity screen (set2)

    Plasmid kit
    #1000000145
    Purpose
    UbiGate E2 Activity Screen (set 2) includes expression cassettes containing ubiquitin, the E1 UBA1 in combination with all Arabidopsis thaliana E2's for E3 autoubiquitination assays. See Kit #1000000144 for Set 1 components.
    Depositor
    Marco Trujillo
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. Cultivarium JERBOA Toolkit

    Plasmid kit
    #1000000275
    Purpose
    Golden Gate genetic toolkit developed to help researchers identify functional transposons and establish genetic tractability in diverse non-model bacteria.
    Depositor
    Nili Ostrov
    Application
    Barcoding, Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    March 12, 2026
    Availability
    Academic Institutions and Nonprofits only

  18. 4G-Cloning Plasmid Kit

    Plasmid kit
    #1000000252
    Purpose
    Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.
    Depositor
    Stephan Gruber
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 2721 - 2740 of 5788 results