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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,692 results for: golden gate

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Showing: 2721 - 2740 of 5692 results

  3. pKLV2-U6-crRNA-BbsI-PGKpuroBFP-W

    Plasmid
    #260192
    Purpose
    Lentiviral backbone to clone Cas3 crRNAs into with BFP expression and puromycin resistance
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Available since
    Sept. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    260192-plasmid-map-sequence-id-521646

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  7. pLenti-Puro-T2A-mCherry

    Plasmid
    #260208
    Purpose
    Lentiviral backbone to clone pegRNA with puromycin resistance and mCherry expression
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    mCherry
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    260208-plasmid-map-sequence-id-520980

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  8. pLenti-ePegRNA-acceptor-BFP

    Plasmid
    #260210
    Purpose
    Lentiviral backbone to clone pegRNA with puromycin resistance and BFP expression, encodes for mRFP for cloning selection
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    BFP
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    260210-plasmid-map-sequence-id-520975

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  9. pLenti-puro-mCherry-epeg

    Plasmid
    #260211
    Purpose
    Lentiviral backbone to clone pegRNA with tevopepQ and puromycin resistance and mCherry expression
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    mCherry
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    260211-plasmid-map-sequence-id-520974

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  10. pGKTM2SP00619

    Plasmid
    #258607
    Purpose
    Level 2 intron-containing mCherry retrotransposition reporter flanked by wt R2Tg 5'/3' UTRs; TMV 5' UTR; R33/R4 rDNA homology arms; 3' HDV ribozyme; pCsB spectinomycin-resistant plant binary vector
    Depositor
    Gözde Demirer
    Publication
    Muchenje et al Nat Biotechnol. 2026 Jun 19. doi: 10.103
    Insert
    pVmv-R33-wt R2Tg 5′ UTR-t35s-mCherry (A. thaliana intron)-TMV 5′ UTR-p35s-wt R2Tg 3′ UTR-R4-HDV-tHSP
    Tags
    Bipartite nuclear localization sequence
    Expression
    Plant
    Promoter
    pVmv
    Available since
    Sept. 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    258607-plasmid-map-sequence-id-520567

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  11. pHW2133

    Plasmid
    #255972
    Purpose
    N-terminal TAG-IN donor plasmid for fusion tagging with 3X HA turboID (selection marker, SEC flanked by Lox511I)
    Depositor
    Han Wang
    Insert
    FRT-SEC(pDD364)-3X HA turboID-N' linker-SD-rFRT3
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255972-plasmid-map-sequence-id-511475

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  14. T2(2)-ori

    Plasmid
    #216627
    Purpose
    Knockout vector for Bacillus velenzensis
    Depositor
    Adam Arkin
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    July 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    216627-plasmid-map-sequence-id-431896

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  15. pGKTM2SP00414

    Plasmid
    #258605
    Purpose
    Level 2 all-in-one R2 editor: R2Tg (T. guttata R2 retroelement, 2 introns); intron-containing mCherry retrotransposition reporter; mTagBFP2 marker; pCsB spectinomycin-resistant plant binary vector.
    Depositor
    Gözde Demirer
    Publication
    Muchenje et al Nat Biotechnol. 2026 Jun 19. doi: 10.103
    Inserts
    pVmv-R33-wt R2Tg 5′ UTR-t35s-mCherry (A. thaliana intron)-TMV 5′ UTR-p35s-wt R2Tg 3′ UTR-R4-HDV-tHSP
    pCmY-R2Tg[+2 A. thaliana introns]-tHSP
    pCmY-mTagBFP2-tHSP
    Tags
    Bipartite nuclear localization sequence, bipartit…
    Expression
    Plant
    Promoter
    pCmY and pVmv
    Available since
    July 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    258605-plasmid-map-sequence-id-516340

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  16. pGKTM1KN00431

    Plasmid
    #258606
    Purpose
    Level 1 R2Tg (T. guttata R2 retroelement) expression cassette with two A. thaliana introns; pCmYLCV6 promoter; A. thaliana HSP18.2 terminator; pCk3 kanamycin-resistant plant expression binary vector.
    Depositor
    Gözde Demirer
    Publication
    Muchenje et al Nat Biotechnol. 2026 Jun 19. doi: 10.103
    Insert
    pCmY-R2Tg[+2 A. thaliana introns]-tHSP
    Tags
    bipartite nuclear localization sequence
    Expression
    Plant
    Promoter
    pCmY
    Available since
    July 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    258606-plasmid-map-sequence-id-515706

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  17. pMB.PRC2-AEBP2LTEV-JARID2

    Plasmid
    #247017
    Purpose
    Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4, AEBP2LTEV (AEBP2 long isoform that can be converted to an AEBP2 short isoform by TEV cleavage), MBP-JARID2 in insect cells, MBP tags are C3-cleav
    Depositor
    Chen Davidovich
    Publication
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Inserts
    EZH2 (EZH2 Human)
    SUZ12 (SUZ12 Human)
    EED (EED Human)
    RBBP4 (RBBP4 Human)
    AEBP2LTEV (AEBP2 Human)
    JARID2 (JARID2 Human)
    Tags
    MBP
    Expression
    Insect
    Mutation
    AEBP2 long isoform that can be converted to an AE…
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    247017-plasmid-map-sequence-id-513523

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  18. Ef1a-NLS-Gal4VP64TF-T2A-mCherry (MC) (pSS085)

    Plasmid
    #236353
    Purpose
    Second generation lentivirus compatible transfer vector encoding an GAL4 and an mCherry transduction marker
    Depositor
    Hana El-Samad
    Insert
    Ef1a-NLS-Gal4VP64TF-T2A-mCherry
    Use
    Lentiviral
    Promoter
    Ef1a
    Available since
    June 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    236353-plasmid-map-sequence-id-489665

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  19. MoClo N-Terminal and C-Terminal Tag Kit

    Plasmid kit
    #1000000277
    Purpose
    A MoClo collection containing a selection of N-Terminal and C-Terminal tag modules, common plant promoters and terminators, and acceptor vectors.
    Depositor
    Mark Youles
    Application
    Cloning and Synthetic Biology
    Vector type
    Plant Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    June 8, 2026
    Availability
    Academic Institutions and Nonprofits only
Showing: 2721 - 2740 of 5692 results