We narrowed to 150,479 results for: ins
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Plasmid#80413PurposeExpresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
ExpressionMammalianAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-EEA1
Plasmid#174451PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
Tagsearly endosomal antigen 1 (EEA1)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-EEA1
Plasmid#174453PurposeExpress iRFP with early endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with early endosome-targeting sequence
Tagsearly endosomal antigen 1 (EEA1)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-Rab11
Plasmid#174462PurposeExpress iRFP with recycling endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with recycling endosome-targeting sequence
Tagssmall GTPase (Rab11)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
ExpressionBacterialPromoterPBADAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)
Plasmid#185918PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-Rab5
Plasmid#174456PurposeExpress iRFP with early endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with early endosome-targeting sequence
Tagssmall GTPase (Rab5)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRExpressionBacterialPromoterP3 constitutive promoter and native promoterAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-mCherry
Plasmid#175829PurposeExpress mCherry with microtubule-targeting sequence in mammalian cellsDepositorInsertmCherry with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI_AFF-1-FLAG_H2B-RFP
Plasmid#240506PurposeCell fusion assaysDepositorInsertAFF-1
TagsH2B-RFPExpressionMammalianAvailable SinceJuly 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-CIAO2-TVA-mCherry
Plasmid#149297PurposeCre-dependent expression of TVA:mCherry fusion protein for rabies tracing.DepositorInsertTVA:mCherry
UseAAVMutationStart codon removed.Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab7
Plasmid#174457PurposeExpress EGFP with late endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with late endosome-targeting sequence
Tagssmall GTPase (Rab7)ExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMW41: pET-His-MBP-SUMO-R2Bm-SII
Plasmid#209290PurposeExpresses the R2Bm ORF in E. coliDepositorInsertR2
TagsHis, MBP, StrepII, and bdSUMOExpressionBacterialPromoterT7Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab5
Plasmid#174454PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
Tagssmall GTPase (Rab5)ExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_MCP-RecT
Plasmid#164803Purposemammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLSDepositorInsertRecT
ExpressionMammalianAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-Rab7
Plasmid#174459PurposeExpress iRFP with late endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with late endosome-targeting sequence
Tagssmall GTPase (Rab7)ExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-EGFP
Plasmid#175827PurposeExpress EGFP with microtubule-targeting sequence in mammalian cellsDepositorInsertEGFP with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only